Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Animal Infectious Disease Unit, National State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture, Wuhan, China; Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
Vaccine. 2018 Dec 18;36(52):8079-8083. doi: 10.1016/j.vaccine.2018.10.071. Epub 2018 Nov 13.
Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and human erysipeloid. Bacterial surface proteins are promising vaccine candidates. We recently identified 3 E. rhusiopathiae surface proteins (GAPDH, HP0728, and HP1472) and characterized their roles as virulence factors. However, their efficacy as protective antigens is still unknown. The N-terminal region of a previously identified surface protein, CbpB (CbpB-N), is speculated to be a protective antigen, but this needs to be verified. The aim of this study was to evaluate the protective efficacy of GAPDH, HP0728, HP1472, and CbpB-N. Immunization with recombinant GAPDH provided complete protection in a mouse model, recombinant CbpB-N provided partial protection, while recombinant HP0728 and HP1472 provided no protection. Recombinant GAPDH also provided good protection in a pig model. GAPDH antiserum exhibited significant blood bactericidal activity against E. rhusiopathiae. In conclusion, GAPDH and CbpB-N were found to be protective antigens of E. rhusiopathiae, and GAPDH is a promising vaccine candidate.
红斑丹毒丝菌是动物丹毒和人类类丹毒的病原体。细菌表面蛋白是有前途的疫苗候选物。我们最近鉴定了 3 种红斑丹毒丝菌表面蛋白（GAPDH、HP0728 和 HP1472），并研究了它们作为毒力因子的作用。然而，它们作为保护性抗原的功效尚不清楚。之前鉴定的表面蛋白 CbpB（CbpB-N）的 N 端区域被推测为保护性抗原，但这需要验证。本研究旨在评估 GAPDH、HP0728、HP1472 和 CbpB-N 的保护效力。用重组 GAPDH 免疫可在小鼠模型中提供完全保护，重组 CbpB-N 提供部分保护，而重组 HP0728 和 HP1472 则没有提供保护。重组 GAPDH 在猪模型中也提供了良好的保护。GAPDH 抗血清对红斑丹毒丝菌表现出显著的血液杀菌活性。总之，发现 GAPDH 和 CbpB-N 是红斑丹毒丝菌的保护性抗原，GAPDH 是一种很有前途的疫苗候选物。
