Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium.
Infect Immun. 1970 Dec;2(6):698-704. doi: 10.1128/iai.2.6.698-704.1970.
Induction of circulating interferon and protection against vaccinia virus infection in mice by injection of Brucella abortus were studied. It was demonstrated that morphologically intact brucellae (either live or killed by heat or exposure to NaOH) induce high and prolonged levels of circulating interferon in mice. In each instance, the inducing principle remained associated with the bacterial particle. Disruption of brucellae by mechanical means destroyed the interferon-inducing capacity. However, by alkalinization of the water extract of disrupted bacilli, an interferon inducer could be rescued. On intravenous injection, this inducer caused a typical endotoxin type of interferon response with a peak value at 2 hr. Mice pretreated with cycloheximide showed an enhanced interferon response to the brucella extract, but a reduced reaction to live brucellae. The significance of these data, in relation to the triggering of de novo interferon synthesis by brucella, is discussed. It was also observed that small doses of brucellae protected mice for at least 1 month against vaccinia virus infection. High doses of heat-or alkali-killed brucellae protected the animals for only a short time, and disrupted brucellae did not afford any protection. Thus, there was a good correlation between interferon-inducing capacity and short-term protective activity. Long-term protection, on the other hand, seemed to be related to multiplication of brucellae in the body.
研究了注射流产布鲁氏菌诱导循环干扰素和预防牛痘病毒感染的作用。结果表明，形态完整的布鲁氏菌（无论是活的还是通过加热或暴露于 NaOH 杀死的）可诱导小鼠体内产生高水平和长时间的循环干扰素。在每种情况下，诱导因子都与细菌颗粒相关联。通过机械手段破坏布鲁氏菌会破坏其诱导干扰素的能力。然而，通过碱化破碎杆菌的水提取物，可以回收一种干扰素诱导剂。静脉注射这种诱导剂会引起典型的内毒素型干扰素反应，2 小时达到峰值。用环己亚胺预处理的小鼠对布鲁氏菌提取物表现出增强的干扰素反应，但对活布鲁氏菌的反应降低。这些数据与布鲁氏菌触发新合成干扰素的关系，值得进一步讨论。此外，还观察到小剂量的布鲁氏菌可使小鼠至少在 1 个月内免受牛痘病毒感染。高热或强碱杀死的布鲁氏菌高剂量仅能提供短暂的保护，而破碎的布鲁氏菌则不能提供任何保护。因此，干扰素诱导能力与短期保护活性之间存在良好的相关性。另一方面，长期保护似乎与布鲁氏菌在体内的繁殖有关。
