Loewen K G, Derbyshire J B
Can J Vet Res. 1986 Apr;50(2):232-7.
Newborn piglets were treated with various doses of polyinosinic:polycytidylic acid intravenously and their serum interferon responses determined by a plaque reduction assay with vesicular stomatitis virus in Madin-Darby bovine kidney cells. A single dose of 5 mg of polyinosinic:polycytidylic acid was found consistently to induce detectable levels of interferon in serum, while the response to lower doses was inconsistent and higher doses produced clinical signs of toxicity. Piglets receiving 5 mg of polyinosinic:polycytidylic acid had maximum serum interferon titers between four and eight hours after treatment, and interferon was no longer detected at 72 hours after treatment. Following treatment with polyinosinic:polycytidylic acid leukopenia was observed, coincident with peak serum interferon titers. Elevated levels of serum glutamic oxaloacetic transaminase and blood urea, indicative of hepatic and renal dysfunction respectively, were also observed following interferon induction with polyinosinic:polycytidylic acid. Piglets treated with polyinosinic:polycytidylic acid also demonstrated antiviral activity in their intestinal mucosal tissues and intestinal washes, but the antiviral activity in the intestinal wash was not characterizable as interferon. A factor in the intestinal washes from newborn piglets was found to antagonize the antiviral effects of interferon by enhancing the plaque forming ability of vesicular stomatitis virus.
聚胞苷酸，并通过在马-达二氏牛肾细胞中采用水疱性口炎病毒蚀斑减少试验来测定它们的血清干扰素反应。发现单次注射5毫克聚肌苷酸:聚胞苷酸能持续诱导血清中可检测到的干扰素水平，而对较低剂量的反应则不一致，较高剂量会产生毒性的临床症状。接受5毫克聚肌苷酸:聚胞苷酸的仔猪在治疗后4至8小时血清干扰素滴度最高，治疗72小时后不再检测到干扰素。用聚肌苷酸:聚胞苷酸治疗后观察到白细胞减少，与血清干扰素滴度峰值同时出现。在用聚肌苷酸:聚胞苷酸诱导干扰素后，还观察到血清谷氨酸草酰乙酸转氨酶和血尿素水平升高，分别表明肝功能和肾功能障碍。用聚肌苷酸:聚胞苷酸治疗的仔猪在其肠道黏膜组织和肠道冲洗液中也表现出抗病毒活性，但肠道冲洗液中的抗病毒活性不能表征为干扰素。发现新生仔猪肠道冲洗液中的一种因子通过增强水疱性口炎病毒的蚀斑形成能力来拮抗干扰素的抗病毒作用。
