Lobund Laboratory, University of Notre Dame, Notre Dame, Indiana 46556.
Infect Immun. 1974 Nov;10(5):1156-62. doi: 10.1128/iai.10.5.1156-1162.1974.
An experimental model was established using the terminal ileum of the rat for characterizing and studying the effects of crude cell-free extract from Clostridium perfringens upon physiological and histological parameters involved in the transport process. Further work was done with the model system using purified enterotoxin (protein) from the cell extract. Using an in vivo perfusion technique it was found that crude extract induces a reversal of net transport, from absorption in controls to secretion, of water, sodium, and chloride. Glucose absorption was greatly inhibited, whereas potassium and bicarbonate transports were unaffected. Crude extract also caused histological damage to the villus epithelium by denuding the villus tips, thereby leaving the lamina propria exposed. Similar responses in transport of water, sodium, chloride, bicarbonate, and glucose were caused by purified toxin. Little or no histological damage resulted from the pure toxin activity. However, the toxin was shown to have the capacity to denude villus tips under the proper experimental conditions.
建立了一个使用大鼠末端回肠的实验模型，用于描述和研究粗无细胞提取物从产气荚膜梭菌对涉及运输过程的生理和组织学参数的影响。进一步使用模型系统用纯化的肠毒素（蛋白）进行了研究。使用体内灌注技术发现，粗提取物诱导净转运的反转，从对照中的吸收到水，钠和氯的分泌。葡萄糖吸收受到极大抑制，而钾和碳酸氢盐的转运不受影响。粗提取物还通过使绒毛尖端裸露而使绒毛上皮组织学损伤，从而使固有层暴露。纯化毒素引起水，钠，氯，碳酸氢盐和葡萄糖转运的类似反应。从纯毒素活性中产生的组织学损伤很少或没有。然而，毒素显示出在适当的实验条件下使绒毛尖端裸露的能力。
