Leonardi C L, Zhu W Y, Kinsey W H, Penneys N S
Veterans' Affairs Medical Center, Department of Dermatology, Miami, FL.
J Cutan Pathol. 1991 Jun;18(3):193-7. doi: 10.1111/j.1600-0560.1991.tb00152.x.
Trichilemmoma is considered a benign neoplasm derived from the outer root sheath of the hair follicle. Although the histogenesis of the lesion is unknown, a relationship between human papillomavirus (HPV) infection and development of these tumors has been suggested on morphologic observations. In order to determine whether HPV is, in fact, present in these lesions, we have analyzed sections from 25 formalin-fixed, paraffin-embedded trichilemmomas for the presence of occult HPV DNA using ultrasensitive DNA amplification technology. Under carefully controlled conditions normally effective in detecting HPV from condyloma or verrucae, we were unable to detect an HPV-induced amplimer in agarose gels or their corresponding Southern blots using radiolabeled probes. Our results do not support the hypothesis that trichilemmomas arise from HPV infection.
毛发上皮瘤被认为是一种源自毛囊外根鞘的良性肿瘤。尽管该病变的组织发生尚不明确，但基于形态学观察，有人提出人类乳头瘤病毒（HPV）感染与这些肿瘤的发生之间存在关联。为了确定HPV是否确实存在于这些病变中，我们使用超灵敏DNA扩增技术，对25例福尔马林固定、石蜡包埋的毛发上皮瘤切片进行分析，以检测隐匿性HPV DNA的存在。在通常能有效从尖锐湿疣或疣中检测出HPV的严格控制条件下，我们无法在琼脂糖凝胶或其相应的用放射性标记探针进行的Southern印迹中检测到HPV诱导的扩增产物。我们的结果不支持毛发上皮瘤由HPV感染引起的假说。
