用于人乳头瘤病毒6、11、16、18、31和33型早期区域1、6和7的DNA扩增的寡核苷酸引物。
Oligonucleotide primers for DNA amplification of the early regions 1, 6, and 7 from human papillomavirus types 6, 11, 16, 18, 31, and 33.
作者信息
Evander M, Bodén E, Bjersing L, Rylander E, Wadell G
机构信息
Department of Virology, University of Umeå, Sweden.
出版信息
Arch Virol. 1991;116(1-4):221-33. doi: 10.1007/BF01319244.
Human papillomavirus (HPV) type-specific sequences required for polymerase chain reaction (PCR) mediated amplification of HPV DNA sequences are presented. One primer pair within the E1 open reading frame (ORF) was shared by HPV 6, HPV 11, HPV 16, and HPV 31, whereas the other primer pair within the E1 ORF was specific for HPV 16. Eight primer pairs from the E6 and E7 ORFs specifically detected HPV 6, HPV 16, HPV 18, and HPV 33 sequences. This system has been used for detection of HPV DNA in biopsies, cytological smears and sections of formalin-fixed tissues.
本文介绍了用于聚合酶链反应（PCR）介导的人乳头瘤病毒（HPV）DNA序列扩增所需的HPV型特异性序列。E1开放阅读框（ORF）内的一对引物可用于HPV 6、HPV 11、HPV 16和HPV 31，而E1 ORF内的另一对引物则对HPV 16具有特异性。来自E6和E7 ORF的八对引物可特异性检测HPV 6、HPV 16、HPV 18和HPV 33序列。该系统已用于检测活检组织、细胞学涂片和福尔马林固定组织切片中的HPV DNA。
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