Lennette E T, Apirion D
J Bacteriol. 1971 Dec;108(3):1322-8. doi: 10.1128/jb.108.3.1322-1328.1971.
A mutant strain of Escherichia coli that fails to recover from prolonged (72 hr) starvation also fails to grow at 43 C. Extracts of this mutant strain show an increased ribonuclease II activity as compared to extracts of the parental strain, and stable ribonucleic acid is degraded to a larger extent in this strain during starvation. Ts(+) transductants and revertants were tested for all the above-mentioned phenotypes. All the Ts(+) transductants and revertants tested behaved like the Ts(+) parental strain, which suggests that all the observed phenotypes are caused by a single sts (starvation-temperature sensitivity) mutation. The reversion rate from sts(-) to sts(+) is rather low but is within the range of reversion rates for other single-site mutations. Three-point transduction crosses located this sts mutation between the ilv and rbs genes. The properties of sts(+)/sts(-) merozygotes suggested that the Ts(-) phenotype of this mutation is recessive.
一种无法从长时间（72小时）饥饿状态恢复的大肠杆菌突变株，在43℃时也无法生长。与亲本菌株的提取物相比，该突变株的提取物显示出核糖核酸酶II活性增加，并且在饥饿期间，该菌株中的稳定核糖核酸降解程度更大。对Ts(+)转导子和回复子进行了上述所有表型的测试。所有测试的Ts(+)转导子和回复子的行为都与Ts(+)亲本菌株相似，这表明所有观察到的表型都是由单个sts（饥饿-温度敏感性）突变引起的。从sts(-)到sts(+)的回复率相当低，但在其他单位点突变的回复率范围内。三点转导杂交将这个sts突变定位在ilv和rbs基因之间。sts(+)/sts(-)部分二倍体的特性表明，该突变的Ts(-)表型是隐性的。
