Department of Pediatrics, Harvard Medical School and Children's Hospital Medical Center, Boston, Massachusetts 02115.
Infect Immun. 1970 Jan;1(1):120-7. doi: 10.1128/iai.1.1.120-127.1970.
Of 100 natural isolates of drug-resistant enteric bacteria, 51 were resistant to spectinomycin (Spc) and 46 contained transferable R factors mediating Spc resistance. All Spc(R) R factors mediated streptomycin and bluensomycin resistance and were fi(+) type. Extracts of R-Spc(R) strains adenylated Spc, dihydrospectinomycin, actinamine, streptomycin, and bluensomycin in vitro in the presence of adenosine triphosphate and Mg(++). Results of genetic and biochemical studies support the hypothesis that these reactions are mediated by a single enzyme.
在 100 株耐药肠内细菌的天然分离株中，51 株对大观霉素（Spc）具有耐药性，46 株含有可转移的 R 因子介导 Spc 耐药性。所有 Spc（R）R 因子均介导链霉素和布卢恩霉素耐药性，且均为 fi(+) 型。R-Spc（R）菌株提取物在三磷酸腺苷和 Mg(++)存在的情况下体外腺嘌呤化 Spc、二氢大观霉素、放线胺、链霉素和布卢恩霉素。遗传和生化研究的结果支持这样的假设，即这些反应是由单一酶介导的。
