Levine A J, Ginsberg H S
J Virol. 1967 Aug;1(4):747-57. doi: 10.1128/JVI.1.4.747-757.1967.
Purified fiber antigen of type 5 adenovirus inhibited the multiplication of type 5 adenovirus by 50% when 35 mug of fiber antigen protein was added to 10(6) KB cells in suspension culture. Although the fiber antigen reduced the number of virions adsorbed per cell when a multiplicity of infection of 50,000 plaque-forming units (PFU)/cell was employed, the number of cells infected was not diminished under these conditions. If a low multiplicity of infection (1.1 PFU/cell) was used, viral adsorption was not detectably decreased. The fiber antigen did not reduce the capability of virions to liberate their viral deoxyribonucleic acid (DNA). The biosyntheses of DNA, ribonucleic acid (RNA), and protein were blocked about 20 to 25 hr after the addition of fiber antigen to cultures of uninfected or type 5 adenovirus-infected KB cells. Most of the fiber antigen protein became cell-associated between 22 and 36 hr after it was added to cells. The hexon antigen neither inhibited viral multiplication nor blocked the biosynthesis of DNA, RNA, or protein. Moreover, the hexon did not attach to KB cells. The profound effects of the fiber antigen were not due to the induction of an interferon-like substance, for actinomycin D did not reduce the ability of the fiber to inhibit multiplication of type 1 poliovirus.
当向悬浮培养的10⁶个KB细胞中加入35微克纤维抗原蛋白时，5型腺病毒的纯化纤维抗原可使5型腺病毒的增殖受到50%的抑制。尽管当感染复数为50,000蚀斑形成单位（PFU）/细胞时，纤维抗原减少了每个细胞吸附的病毒粒子数量，但在这些条件下被感染的细胞数量并未减少。如果使用低感染复数（1.1 PFU/细胞），则病毒吸附未检测到明显下降。纤维抗原并未降低病毒粒子释放其病毒脱氧核糖核酸（DNA）的能力。在向未感染或5型腺病毒感染的KB细胞培养物中加入纤维抗原后约20至25小时，DNA、核糖核酸（RNA）和蛋白质的生物合成被阻断。在将纤维抗原蛋白加入细胞后22至36小时之间，大部分纤维抗原蛋白与细胞结合。六邻体抗原既不抑制病毒增殖，也不阻断DNA、RNA或蛋白质的生物合成。此外，六邻体不附着于KB细胞。纤维抗原的深远作用并非由于诱导了类似干扰素的物质，因为放线菌素D并未降低纤维抑制1型脊髓灰质炎病毒增殖的能力。
