Kirkland J J, Durham N N
Department of Microbiology, Oklahoma State University, Stillwater, Oklahoma.
J Bacteriol. 1965 Jul;90(1):15-22. doi: 10.1128/jb.90.1.15-22.1965.
Kirkland, Jerry J. (Oklahoma State University, Stillwater), and Norman N. Durham. Synthesis of protocatechuate oxygenase by Pseudomonas fluorescens in the presence of exogenous carbon sources. J. Bacteriol. 90:15-22. 1965.-The addition of glucose, ribose, or fructose (0.45 or 45.0 mumoles/ml) simultaneously with protocatechuic acid shortens the lag period required for synthesis of protocatechuate oxygenase by a washed-cell suspension of Pseudomonas fluorescens. Glucose is readily oxidized and supports growth of P. fluorescens, whereas neither ribose nor fructose readily supports growth. High glucose concentrations (45.0 mumoles/ml) shorten the lag period but lower the total enzyme synthesis. The pH drops during glucose oxidation, and this is accompanied by a decrease in the rate of enzyme synthesis. High glucose concentrations, with adequate buffering, permitted "normal" enzyme synthesis. A decrease in the total enzyme synthesis was not observed in the presence of high concentrations of ribose or fructose. Succinate, pyruvate, acetate, or formate (0.45 mumole/ml) were readily oxidized, but did not shorten the lag period required for synthesis of the enzyme. The data suggest that glucose, ribose, or fructose may serve as a "specific" carbon source (such as ribose-5-phosphate or a similar precursor important in ribonucleic acid synthesis) functional in the synthesis of protocatechuate oxygenase.
柯克兰德，杰里·J.（俄克拉荷马州立大学，斯蒂尔沃特），以及诺曼·N. 达勒姆。荧光假单胞菌在外源碳源存在下原儿茶酸加氧酶的合成。《细菌学杂志》90:15 - 22。1965年。——在加入原儿茶酸的同时添加葡萄糖、核糖或果糖（0.45或45.0微摩尔/毫升），可缩短荧光假单胞菌洗涤细胞悬液合成原儿茶酸加氧酶所需的延迟期。葡萄糖易于氧化并支持荧光假单胞菌生长，而核糖和果糖都不易支持其生长。高浓度葡萄糖（45.0微摩尔/毫升）可缩短延迟期，但会降低总酶合成量。葡萄糖氧化过程中pH值下降，同时酶合成速率也降低。高浓度葡萄糖在有足够缓冲的情况下可实现“正常”的酶合成。在高浓度核糖或果糖存在时未观察到总酶合成量减少。琥珀酸盐、丙酮酸盐、乙酸盐或甲酸盐（0.45微摩尔/毫升）易于氧化，但不会缩短合成该酶所需的延迟期。数据表明，葡萄糖、核糖或果糖可能作为一种“特定”碳源（如5 - 磷酸核糖或在核糖核酸合成中重要的类似前体）在原儿茶酸加氧酶的合成中起作用。
