FREUNDLICH M, LICHSTEIN H C
J Bacteriol. 1962 Nov;84(5):988-95. doi: 10.1128/jb.84.5.988-995.1962.
Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. II. Effect of glucose. J. Bacteriol. 84:988-995. 1962.-The effect of glucose and other compounds on the formation of tryptophanase and tryptophan synthetase in Escherichia coli was examined. Although most of these compounds were potent inhibitors of the synthesis of tryptophanase, they invariably increased the formation of tryptophan synthetase. The severity of tryptophanase inhibition depended upon the degree of utilization of the compound by the growing bacterial cells. It was found that high levels of tryptophan overcame by 40% the repression caused by glucose. The stimulatory effect of glucose on tryptophan synthetase formation in E. coli 9723E could be duplicated by indole-3-propionic acid. A study of the amino acid pool of E. coli 9723E revealed no free tryptophan in cells harvested from the basal medium containing glucose. In contrast, cells grown in the absence of glucose possessed a measurable amount of this amino acid. The possible mechanisms of the effect of glucose and related compounds on tryptophanase and tryptophan synthetase formation, as well as the relationship of these effects to the metabolic control of tryptophan metabolism, are discussed.
弗罗因德利希，马丁（明尼苏达大学，明尼阿波利斯）和赫尔曼·C·利希斯坦。大肠杆菌中的色氨酸酶-色氨酸合成酶系统。II. 葡萄糖的影响。《细菌学杂志》84:988 - 995。1962年。——研究了葡萄糖和其他化合物对大肠杆菌中色氨酸酶和色氨酸合成酶形成的影响。尽管这些化合物大多是色氨酸酶合成的强效抑制剂，但它们总是会增加色氨酸合成酶的形成。色氨酸酶抑制的严重程度取决于生长中的细菌细胞对该化合物的利用程度。发现高水平的色氨酸可克服40%由葡萄糖引起的阻遏作用。葡萄糖对大肠杆菌9723E中色氨酸合成酶形成的刺激作用可用吲哚-3-丙酸来复制。对大肠杆菌9723E氨基酸库的研究表明，从含有葡萄糖的基础培养基中收获的细胞中没有游离色氨酸。相比之下，在无葡萄糖条件下生长的细胞含有可测量量的这种氨基酸。讨论了葡萄糖及相关化合物对色氨酸酶和色氨酸合成酶形成的影响的可能机制，以及这些影响与色氨酸代谢的代谢控制之间的关系。
