Michnovicz J J, Bradlow H L
Institute for Hormone Research, New York, NY 10016.
Nutr Cancer. 1991;16(1):59-66. doi: 10.1080/01635589109514141.
Research studies have demonstrated a strong association between estrogen metabolism and the incidence of breast cancer, and we have therefore sought pharmacological means of favorably altering both metabolism and subsequent risk. Indole-3-carbinol (I3C), obtained from cruciferous vegetables (e.g., cabbage, broccoli, etc.), is a known inducer of oxidative P-450 metabolism in animals. We investigated the effects in humans of short-term oral exposure to this compound (6-7 mg/kg/day over 7 days). We used an in vivo radiometric test, which provided a highly specific and reproducible measure of estradiol 2-hydroxylation before and after exposure to I3C. In a group of 12 healthy volunteers, the average extent of reaction increased by approximately 50% during this short exposure (p less than 0.01), affecting men and women equally. We also measured the urinary excretion of two key estrogen metabolites, 2-hydroxyestrone (2OHE1) and estriol (E3). We found that the excretion of 2OHE1 relative to that of E3 was significantly increased by I3C, further confirming the ongoing induction of 2-hydroxylation. These results indicate that I3C predictably alters endogenous estrogen metabolism toward increased catechol estrogen production and may thereby provide a novel "dietary" means for reducing cancer risk.
研究表明，雌激素代谢与乳腺癌发病率之间存在密切关联，因此我们一直在寻找能有利改变代谢及后续风险的药理学方法。吲哚 - 3 - 甲醇（I3C）可从十字花科蔬菜（如卷心菜、西兰花等）中获取，是一种已知的动物体内氧化型P - 450代谢诱导剂。我们研究了人类短期口服该化合物（7天内每天6 - 7毫克/千克）的效果。我们采用了一种体内放射性测试，该测试能在接触I3C前后对雌二醇2 - 羟化作用提供高度特异性且可重复的测量。在一组12名健康志愿者中，在这种短期接触期间反应的平均程度增加了约50%（p小于0.01），对男性和女性的影响相同。我们还测量了两种关键雌激素代谢物2 - 羟基雌酮（2OHE1）和雌三醇（E3）的尿排泄量。我们发现，I3C使2OHE1相对于E3的排泄量显著增加，进一步证实了2 - 羟化作用的持续诱导。这些结果表明，I3C可预期地改变内源性雌激素代谢，使其朝着增加儿茶酚雌激素生成的方向发展，从而可能提供一种降低癌症风险的新型“饮食”方法。
