Anagnostou Fani, Debet Aurore, Pavon-Djavid Graciela, Goudaby Zakaryia, Hélary Gérard, Migonney Véronique
Laboratoire de Recherches Orthopediques, Biomécanique et Biomatériaux Osteo-articulaires, UMR C.N.R.S. 7052, 10, avenue de Verdun, 75015 Paris, France.
Biomaterials. 2006 Jul;27(21):3912-9. doi: 10.1016/j.biomaterials.2006.03.004. Epub 2006 Mar 27.
Staphylococcus aureus adhesion and osteoblast functions were assessed on functionalized poly(methyl methacrylate)-based terpolymers bearing randomly distributed carboxylate and sulfonate groups. These terpolymers were synthesized by radical polymerization, characterized by nuclear resonance spectroscopy and classified by the ratio R=[COO(-)/COO(-)+SO(3)(-)] in the range 0.5-0.8. Bacterial adhesion study showed that fibronectin-coated terpolymers with R varying from 0.5 to 0.8 exhibited inhibition rate of S. aureus adhesion from 90% to 98% as compared to the adhesion on unfunctionalized poly(methyl methacrylate). In contrast, the adhesion of osteoblasts onto the same functionalized terpolymers was decreased by 20% when compared to the results obtained on poly(methyl methacrylate). While the amount of attached osteoblasts are similar onto all the functionalized terpolymers whatever its R value, the cell proliferation was different and was found to vary with R in the range 0.5-0.8. Osteoblast proliferation, alkaline phosphatase activity and accumulation of calcium in the extracellular matrix of these cells, cultured on the functionalized terpolymers with R equal to 0.7-0.8 were similar to that observed onto non-functionalized poly(methyl methacrylate). In contrast, osteoblast proliferation was inhibited on terpolymers with an R value around 0.6. These results provide evidence that functionalized poly(methyl methacrylate)-based terpolymers with R ratio equaling 0.7-0.8 simultaneously inhibit bacteria adhesion and support osteoblast functions pertinent to new bone formation. These functionalized polymers could, therefore, be used as coating or grafted on orthopedic and dental implants to render them both bone compatible and able to prevent bacterial infection.
在带有随机分布的羧基和磺酸根基团的功能化聚甲基丙烯酸甲酯基三元共聚物上评估了金黄色葡萄球菌的黏附及成骨细胞功能。这些三元共聚物通过自由基聚合合成，采用核磁共振光谱进行表征，并根据比率R = [COO(-)/COO(-)+SO(3)(-)]在0.5 - 0.8范围内进行分类。细菌黏附研究表明，与未功能化的聚甲基丙烯酸甲酯上的黏附相比，R值在0.5至0.8之间的纤连蛋白包被的三元共聚物对金黄色葡萄球菌黏附的抑制率为90%至98%。相比之下，与在聚甲基丙烯酸甲酯上获得的结果相比，成骨细胞在相同功能化三元共聚物上的黏附减少了20%。虽然无论R值如何，附着在所有功能化三元共聚物上的成骨细胞数量相似，但细胞增殖情况不同，且发现其随R值在0.5 - 0.8范围内变化。在R值等于0.7 - 0.8的功能化三元共聚物上培养的这些细胞，其成骨细胞增殖、碱性磷酸酶活性以及细胞外基质中钙的积累与在未功能化聚甲基丙烯酸甲酯上观察到的情况相似。相反，在R值约为0.6的三元共聚物上成骨细胞增殖受到抑制。这些结果表明，R比率等于0.7 - 0.8的功能化聚甲基丙烯酸甲酯基三元共聚物可同时抑制细菌黏附并支持与新骨形成相关的成骨细胞功能。因此，这些功能化聚合物可用作涂层或接枝到骨科和牙科植入物上，使其既具有骨相容性又能预防细菌感染。
