Department of Prosthodontics, Institute of Stomatology & Research Center of Dental Esthetics and Biomechanics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
Department of Prosthodontics, Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Laboratory of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, People's Republic of China.
Int J Nanomedicine. 2024 Nov 1;19:11143-11159. doi: 10.2147/IJN.S482478. eCollection 2024.
The study was intended to compare the surface properties and the bacterial and biofilm adhesion resistance of two potential antibacterial nanometer titanium dioxide (nano-TiO) coatings on dental titanium (Ti) abutments prepared by atomic layer deposition (ALD) and the anodic oxidation (AO) techniques.
Nano-TiO₂ coatings were developed using ALD and AO techniques and applied to Ti surfaces. The surface properties and the bacterial and biofilm adhesion resistance of these coatings were evaluated against commonly used Ti and Zirconia (ZrO₂) surfaces. The chemical compositions, crystalline forms, surface topography, roughness and hydrophilicity were characterized. The antibacterial performance was assessed by the scanning electron microscope (SEM), the Colony-forming unit (CFU) assay and the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay using in vitro models of ( ), ( ), and ( ) in both single- and mixed-species bacterial compositions.
ALD-prepared nano-TiO₂ coatings resulted in a dense, smooth, and less hydrophilic surface with an anatase phase, significantly reducing the adhesion of the three bacteria by over 50%, comparable to ZrO₂. In contrast, AO-prepared coatings led to a less hydrophilic surface, characterized by various nano-sized pores within the oxide film. This alteration, however, had no impact on the adhesion of the three bacteria. The adhesion patterns for mixed-species bacteria were generally consistent with single-species results.
ALD-prepared nano-TiO₂ coatings on Ti abutments demonstrated promising antibacterial properties comparable to ZrO₂ surfaces, suggesting potential in preventing peri-implantitis. However, the bacterial and biofilm adhesion resistance of AO-produced nano-TiO₂ coatings was limited.
本研究旨在比较原子层沉积（ALD）和阳极氧化（AO）技术制备的两种潜在抗菌纳米二氧化钛（nano-TiO₂）涂层在牙科钛（Ti）基台上的表面性能以及细菌和生物膜黏附阻力。
使用 ALD 和 AO 技术制备 nano-TiO₂涂层并应用于 Ti 表面。评估这些涂层对常用 Ti 和氧化锆（ZrO₂）表面的表面性能以及细菌和生物膜黏附阻力。通过扫描电子显微镜（SEM）、集落形成单位（CFU）测定和 3-（4,5-二甲基噻唑基-2）-2,5-二苯基四氮唑溴盐（MTT）测定评估抗菌性能，采用体外模型评估单一和混合物种细菌组成中 、 、 的单种和混合种细菌组成。
ALD 制备的 nano-TiO₂涂层导致表面致密、光滑且亲水性降低，呈锐钛矿相，显著降低了三种细菌的黏附率超过 50%，与 ZrO₂相当。相比之下，AO 制备的涂层导致表面亲水性降低，氧化物膜内具有各种纳米级孔。然而，这种变化对三种细菌的黏附没有影响。混合种细菌的黏附模式通常与单种细菌的结果一致。
Ti 基台上 ALD 制备的 nano-TiO₂涂层表现出与 ZrO₂表面相当的有前景的抗菌性能，表明有潜力预防种植体周围炎。然而，AO 产生的 nano-TiO₂涂层的细菌和生物膜黏附阻力有限。
