Williams Philip, White Andrew, Ferraro Damien, Clark Suzanne, Staley Kevin, Dudek F Edward
Department of Biomedical Sciences, Anatomy and Neurobiology Section, Colorado State University, Fort Collins, CO 80523, USA.
J Neurosci Methods. 2006 Jul 15;155(1):39-48. doi: 10.1016/j.jneumeth.2005.12.035. Epub 2006 Mar 27.
Temporal lobe epilepsy in humans is a chronic condition with a highly variable temporal evolution. Animal models of this disorder have been developed to recapitulate many of the characteristics seen in humans with temporal lobe epilepsy. These animal models generate chronic spontaneous electrographic and motor seizures with a progressive increase in frequency over many months. In order to understand the underlying cellular and molecular mechanisms driving epileptogenesis, a practical means for accurately assessing seizure progression over this extended time period must be devised. In this report, we describe the use of a three-channel radiotelemetry system to record spontaneous electrographic interictal "spikes" and seizure activity from the cortical surface and the two hippocampi. This approach has allowed continuous recording before, during, and several months after kainate-induced status epilepticus. The important advantages of this approach are the potential for long-term continuous electrographic recording with comparatively unrestricted behavior; the disadvantages include increased cost, surgical difficulty and lower frequency-response in the recordings.
人类颞叶癫痫是一种慢性疾病，其时间演变高度可变。已经开发出这种疾病的动物模型，以重现人类颞叶癫痫中出现的许多特征。这些动物模型会产生慢性自发性脑电图和运动性癫痫发作，且在数月内发作频率逐渐增加。为了了解驱动癫痫发生的潜在细胞和分子机制，必须设计出一种在这段较长时间内准确评估癫痫发作进展的实用方法。在本报告中，我们描述了使用一种三通道无线电遥测系统来记录皮质表面和两个海马体的自发性脑电图发作间期“尖峰”和癫痫发作活动。这种方法能够在海藻酸诱导的癫痫持续状态之前、期间以及之后的几个月进行连续记录。这种方法的重要优点是有可能进行长期连续脑电图记录，且行为相对不受限制；缺点包括成本增加、手术难度加大以及记录中的频率响应较低。
