Woolston C J, Covey S N, Penswick J R, Davies J W
Gene. 1983 Jul;23(1):15-23. doi: 10.1016/0378-1119(83)90212-3.
Infection of young turnip leaves with an aphid-transmissible isolate, Cabb B-JI, of cauliflower mosaic virus (CaMV) causes synthesis of an Mr 18 000 polypeptide (p18) which co-purifies with virus inclusion bodies. This polypeptide is not detectable in leaves infected with either of two aphid non-transmissible isolates. Campbell and CM4-184. Construction in vitro, of hybrid genomes between Cabb B-JI and Campbell isolates demonstrates that aphid transmissibility and presence of p18 is dependent on the small genome fragment from the BstEII site to the XhoI site. A deletion made in this fragment within open reading frame (ORF) II causes loss of aphid transmissibility and also terminates production of p18. We conclude that aphid transmissibility and the presence of p18 are related to the expression of ORF II of the CaMV genome.
用芜菁花叶病毒（CaMV）的一种蚜虫传播分离株Cabb B-JI感染幼嫩芜菁叶，会导致合成一种分子量为18000的多肽（p18），该多肽与病毒包涵体共纯化。在用两种蚜虫非传播分离株Campbell和CM4-184感染的叶片中检测不到这种多肽。在体外构建Cabb B-JI和Campbell分离株之间的杂交基因组表明，蚜虫传播性和p18的存在取决于从BstEII位点到XhoI位点的小基因组片段。在开放阅读框（ORF）II内的这个片段中进行的缺失导致蚜虫传播性丧失，也终止了p18的产生。我们得出结论，蚜虫传播性和p18的存在与CaMV基因组ORF II的表达有关。
