Borisova Ekaterina, Uzunov Tzonko, Avramov Latchezar
Institute of Electronics, Bulgarian Academy of Sciences, 72, Tsarigradsko Shossee, Blvd, 1784, Sofia, Bulgaria.
Lasers Med Sci. 2006 Apr;21(1):34-41. doi: 10.1007/s10103-005-0365-7. Epub 2006 Mar 28.
Laser-induced autofluorescence spectra of teeth irradiated by a 337 nm nitrogen laser were measured during in vitro caries formation through initial enamel demineralization and introducing of carious bacterial flora in the lesions developed. Spectra obtained from sound teeth consist of an intensive maximum at 480-500 nm and secondary maximum at 430-450 nm. In the process of caries formation, we observed an increase in the intensity at 430-450 nm and the appearance of two maxima in the red spectral region-at 590-650 nm. The intensity increase at 430-450 nm was related to the tooth demineralization. Bacteria presence and their metabolism products induced an increase in the absorption in the UV-blue spectral region at 350-420 nm and the appearance of a fluorescence signal in the long-wave spectral region at 590-650 nm. From the point of view of tissue optics, these results allow caries to be considered as consisting of two different phenomena-tissue destruction and bacterial flora and its metabolism products increase. The results could be used to obtain a more complete picture of caries formation on the base of its fluorescent properties.
在体外龋齿形成过程中，通过初始釉质脱矿以及在形成的病变中引入龋细菌菌群，测量了337nm氮激光照射牙齿的激光诱导自体荧光光谱。从健康牙齿获得的光谱在480 - 500nm处有一个强烈的最大值，在430 - 450nm处有一个次要最大值。在龋齿形成过程中，我们观察到430 - 450nm处的强度增加，并且在红色光谱区域（590 - 650nm）出现了两个最大值。430 - 450nm处的强度增加与牙齿脱矿有关。细菌的存在及其代谢产物导致350 - 420nm的紫外 - 蓝色光谱区域吸收增加，以及在590 - 650nm的长波光谱区域出现荧光信号。从组织光学的角度来看，这些结果使得龋齿可以被认为是由两种不同的现象组成——组织破坏以及细菌菌群及其代谢产物增加。这些结果可用于基于龋齿的荧光特性获得更完整的龋齿形成情况。
