人IgG亚类抗体在异盘并殖吸虫病血清学诊断中的评价

Evaluation of human IgG subclass antibodies in the serodiagnosis of Paragonimiasis heterotremus.

作者信息

Wongkham Chaisiri, Intapan Pewpan M, Maleewong Wanchai, Miwa Masanao

机构信息

Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Thailand.

出版信息

Asian Pac J Allergy Immunol. 2005 Dec;23(4):205-11.

PMID:16572740

Abstract

Immunoglobulin G subclass antibodies (IgG1, IgG2, IgG3, and IgG4) responses to the excretory-secretory antigens of the lung fluke, Paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. Serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. The results indicate that immunoblotting for the detection of IgG4 antibodies to an excretory-secretory product of P. heterotremus of an approximate molecular mass of 31.5 kDa, is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 97.6%, 100%, 96.9%, 90% and 100%, respectively.

摘要

利用免疫印迹技术分析了对异盘肺吸虫排泄-分泌抗原的免疫球蛋白G亚类抗体（IgG1、IgG2、IgG3和IgG4）反应，旨在进一步提高人体肺吸虫病血清学诊断的敏感性和特异性。分析了确诊肺吸虫病患者、其他寄生虫感染患者、肺结核患者以及健康对照者的血清样本。结果表明，检测针对分子量约为31.5 kDa的异盘肺吸虫排泄-分泌产物的IgG4抗体的免疫印迹法是最可靠的检测方法。其准确性、敏感性、特异性以及阳性和阴性预测值分别为97.6%、100%、96.9%、90%和100%。