Kawana M, Kawana C, Yokoo T, Quie P G, Giebink G S
Otitis Media Research Center, University of Minnesota, School of Medicine, Minneapolis 55455.
Infect Immun. 1991 Nov;59(11):4084-8. doi: 10.1128/iai.59.11.4084-4088.1991.
To determine whether oxidative metabolic products of phagocytic cells are present in the middle ear during experimental pneumococcal otitis media, we measured the concentration of myeloperoxidase (MPO) in middle ear fluid (MEF) and the capacity of neutrophils isolated from MEF and peripheral blood to produce MPO and superoxide anion (O2-) after in vitro stimulation. Free MPO in MEF was significantly increased 24 and 48 h after either viable or nonviable pneumococci were inoculated into the middle ear. In vitro-stimulated production of MPO and O2- from middle ear neutrophils was significantly less than that from peripheral blood neutrophils 24 h after nonviable pneumococci were inoculated but similar to it after 48 h. Twenty-four hours after viable pneumococci were inoculated, middle ear neutrophils stimulated in vitro produced less MPO but the same amount of O2- as did blood neutrophils. Oxidative metabolic products, therefore, are released from phagocytic cells into the MEF during pneumococcal otitis media, and future studies will need to define the contribution of these products to acute and chronic middle ear tissue injury.
为了确定在实验性肺炎球菌性中耳炎期间吞噬细胞的氧化代谢产物是否存在于中耳，我们测量了中耳积液（MEF）中髓过氧化物酶（MPO）的浓度，以及从MEF和外周血中分离出的中性粒细胞在体外刺激后产生MPO和超氧阴离子（O2-）的能力。在将活的或灭活的肺炎球菌接种到中耳后24小时和48小时，MEF中的游离MPO显著增加。在接种灭活肺炎球菌24小时后，中耳中性粒细胞体外刺激产生的MPO和O2-明显少于外周血中性粒细胞，但在48小时后与之相似。接种活肺炎球菌24小时后，体外刺激的中耳中性粒细胞产生的MPO较少，但产生的O2-量与血液中性粒细胞相同。因此，在肺炎球菌性中耳炎期间，氧化代谢产物从吞噬细胞释放到MEF中，未来的研究需要确定这些产物对急性和慢性中耳组织损伤的作用。
