Elshiekh N A, Harris-Hamilton E, Bachenheimer S L
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599-7290.
J Virol. 1991 Dec;65(12):6430-7. doi: 10.1128/JVI.65.12.6430-6437.1991.
The time course of accumulation of herpes simplex virus immediate-early (IE) mRNA and the requirement for infected cell protein synthesis for mRNA transcription and accumulation were compared. Measurements of transcription in nuclear run-on assays, accumulation of cytoplasmic mRNA by Northern (RNA) blot hybridization, and rates of infected cell protein synthesis by pulse-labeling did not indicate differences among the five IE gene, consistent with previous studies. However, as a result of varying the amount of de novo protein synthesis after infection, at least three patterns of maximal expression of the IE genes were revealed. Addition of the protein synthesis inhibitor anisomycin to cells coincident with infection resulted in maximal rates of transcription and accumulation of functional ICP0 mRNA, while 0.5 h of infected cell protein synthesis prior to addition of the drug was required for maximal expression of ICP22/47 and ICP27 mRNAs. Maximal expression of ICP4 mRNA occurred only when 1 h of de novo protein synthesis occurred prior to the addition of the drug. These results are discussed in the context of alternative mechanisms for regulating IE gene expression.
比较了单纯疱疹病毒立即早期（IE）mRNA积累的时间进程以及mRNA转录和积累对感染细胞蛋白质合成的需求。通过核转录分析测量转录、通过Northern（RNA）印迹杂交测量细胞质mRNA的积累以及通过脉冲标记测量感染细胞蛋白质合成的速率，结果表明五个IE基因之间没有差异，这与先前的研究一致。然而，由于感染后从头合成蛋白质的量不同，至少揭示了三种IE基因最大表达模式。在感染时向细胞中添加蛋白质合成抑制剂茴香霉素会导致功能性ICP0 mRNA的转录和积累达到最大速率，而在添加药物之前进行0.5小时的感染细胞蛋白质合成是ICP22/47和ICP27 mRNA最大表达所必需的。只有在添加药物之前进行1小时的从头合成蛋白质时，ICP4 mRNA才会出现最大表达。这些结果在调节IE基因表达的替代机制的背景下进行了讨论。
