Wessler S R
Botany Department, University of Georgia, Athens 30602.
Mol Cell Biol. 1991 Dec;11(12):6192-6. doi: 10.1128/mcb.11.12.6192-6196.1991.
The null wx-ml allele contains a 409-bp Dissociation 1 (Ds1) element in exon 9 of the maize waxy (Wx) gene. In the absence of the autonomous Activator (Ac) element, the Ds1 element cannot transpose, and this allele encodes several Wx transcripts that arise following alternative splicing of Ds1 sequences from Wx pre-mRNA. Splicing involves the utilization of three 5' splice sites and three 3' splice sites. All but one of these splice sites are in Ds1 sequences near the ends of the element. The presence of 5' and 3' splice sites near the Ds1 termini and the element's small size and AT richness are features that distinguish Ds1 elements from all other known Ds elements. It is suggested that these features may enhance the ability of Ds1 to function as a mobile intron.
无效的wx - ml等位基因在玉米蜡质(Wx)基因的第9外显子中包含一个409碱基对的解离1(Ds1)元件。在没有自主激活子(Ac)元件的情况下,Ds1元件不能转座,并且该等位基因编码几种Wx转录本,这些转录本是在从Wx前体mRNA中对Ds1序列进行可变剪接后产生的。剪接涉及三个5'剪接位点和三个3'剪接位点的利用。除了其中一个剪接位点外,所有这些剪接位点都位于元件末端附近的Ds1序列中。Ds1末端附近存在5'和3'剪接位点以及该元件的小尺寸和富含AT的特性是将Ds1元件与所有其他已知Ds元件区分开来的特征。有人认为这些特征可能增强Ds1作为移动内含子发挥作用的能力。