Carrera P, Martínez-Balbás M A, Portugal J, Azorín F
Centro de Investigación y Desarrollo-CSIC, Barcelona, Spain.
Nucleic Acids Res. 1991 Oct 25;19(20):5639-44. doi: 10.1093/nar/19.20.5639.
In this paper, the contribution of different sequence elements to the intrisic curvature of the mouse satellite DNA repeat was investigated. This DNA fragment contains nineteen groups of three or more consecutive adenines which are only poorly phased with respect to the helical repeat. The mouse satellite DNA repeat shows a sinusoidal pattern of cleavage by the hydroxyl radical; the waves of reactivity are phased with respect to the A-tracts. Some interesting observations arise from a detailed analysis of these cleavage patterns: a) the maxima of hydroxyl radical cleavage are more periodically spaced along the DNA sequence than the A-tracts themselves. As a consequence, the position of each maximum with respect to the A-tract is variable; b) the sequence 5' TGGAATATG/AA 3' shows a sinusoidal pattern of hydroxyl radical cleavage. This sequence shows a retarded migration in polyacrylamide gels indicating that it is actually intrinsically curved. These results are discussed in view of the current models for DNA curvature.
在本文中，研究了不同序列元件对小鼠卫星DNA重复序列固有曲率的贡献。该DNA片段包含19组三个或更多连续的腺嘌呤，这些腺嘌呤相对于螺旋重复序列的相位很差。小鼠卫星DNA重复序列显示出由羟基自由基切割产生的正弦模式；反应波相对于A序列呈相位关系。对这些切割模式的详细分析产生了一些有趣的观察结果：a）羟基自由基切割的最大值在DNA序列上的间隔比A序列本身更具周期性。因此，每个最大值相对于A序列的位置是可变的；b）序列5'TGGAATATG/AA 3'显示出羟基自由基切割的正弦模式。该序列在聚丙烯酰胺凝胶中迁移滞后，表明它实际上具有固有弯曲。结合当前的DNA曲率模型对这些结果进行了讨论。
