Ohshima K, Kikuchi M, Masuda Y, Sumiyoshi Y, Eguchi F, Mohtai H, Takeshita M, Kimura N
Department of Pathology, School of Medicine, Fukuoka University, Japan.
Acta Pathol Jpn. 1991 Jun;41(6):437-43. doi: 10.1111/j.1440-1827.1991.tb03210.x.
Lymphoepithelioma of the nasopharynx is a neoplasm known to have a strong association with Epstein-Barr virus (EBV). Using the Southern blot method, polymerase chain reaction (PCR), and/or in situ hybridization, we examined lymph nodes containing metastatic carcinoma, including metastatic lymphoepithelioma, for the presence of EBV genomes in order to determine whether EBV was associated exclusively with lymphoepithelioma. All of six lymph nodes from patients with lymphoepithelioma in the neck were found to have EBV genomes using the above methods. In four of the six cases, the primary site was the nasopharynx, and in the other two no primary site was found. Four of 12 squamous cell carcinomas and one of 18 adenocarcinomas expressed the EBV genome only by PCR, but not by Southern blotting or in situ hybridization, probably due to the presence of latent EBV in lymphocytes. These results indicate that metastatic carcinoma in lymph nodes showing EBV genomes revealed by Southern blotting or in situ hybridization is lymphoepithelioma, and that the nasopharynx is very likely the primary site.
鼻咽癌淋巴上皮瘤是一种已知与爱泼斯坦-巴尔病毒（EBV）密切相关的肿瘤。我们使用Southern印迹法、聚合酶链反应（PCR）和/或原位杂交技术，检测了含有转移性癌（包括转移性淋巴上皮瘤）的淋巴结中EBV基因组的存在情况，以确定EBV是否仅与淋巴上皮瘤相关。使用上述方法，发现颈部患有淋巴上皮瘤患者的所有6个淋巴结均含有EBV基因组。6例中有4例的原发部位是鼻咽部，另外2例未发现原发部位。12例鳞状细胞癌中有4例以及18例腺癌中有1例仅通过PCR检测到EBV基因组，而Southern印迹法或原位杂交未检测到，这可能是由于淋巴细胞中存在潜伏性EBV。这些结果表明，通过Southern印迹法或原位杂交检测到含有EBV基因组的淋巴结中的转移性癌是淋巴上皮瘤，并且鼻咽部很可能是原发部位。
