Department of Biochemistry, The Weizmann Institute of Science, Rehovot 76100, Israel.
Proc Natl Acad Sci U S A. 1982 May;79(10):3183-7. doi: 10.1073/pnas.79.10.3183.
External electric field-induced ADP phosphorylation (EFP) in lettuce chloroplasts was monitored with a coupled luciferin-luciferase enzymatic assay. This assay made it possible to follow ATP synthesis in a kinetically competent manner. The EFP reaction was found to be a much slower process than the light-driven reaction in the same system. The amount of ATP synthesized after a single electric field pulse corresponds to many turnovers of the ATP synthase.
用电偶联荧光素酶酶法检测了生菜叶绿体中的 ADP 磷酸化（EFP）。该测定法可使我们以动力学上合适的方式跟踪 ATP 的合成。EFP 反应比同一系统中的光驱动反应慢得多。单个电场脉冲后合成的 ATP 量相当于 ATP 合酶的多次周转。
