二磷酸腺苷（ADP）与光合磷酸化偶联因子之间的相互作用。
Interactions between ADP and the coupling factor of photophosphorylation.
作者信息
Roy H, Moudrianakis E N
出版信息
Proc Natl Acad Sci U S A. 1971 Feb;68(2):464-8. doi: 10.1073/pnas.68.2.464.
Abstract
The coupling factor of photophosphorylation, which carries out the terminal steps in the light-dependent synthesis of ATP in spinach chloroplasts, forms tight complexes with [(14)C]ADP in vitro. The bound [(14)C]ADP undergoes a transphosphorylation reaction to give [(14)C]AMP and [(14)C]ATP. The [(14)C]ATP remains tightly bound, and can be recovered conveniently only by denaturation of the enzyme nucleotide complex. If spinach membranes are illuminated in the presence of pyocyanine and [(3)H]AMP or [(32)P]P(i), the enzyme can be recovered as a tight complex with [(3)H]ADP or [(32)P]ADP. The evidence indicates that AMP is an earlier acceptor of phosphate than is ADP, in a light-driven phosphorylation reaction. It also suggests that AMP serves as a cofactor in photophosphorylation.
摘要
光磷酸化的偶联因子在菠菜叶绿体中依赖光的ATP合成过程中执行终端步骤，它在体外与[（14）C]ADP形成紧密复合物。结合的[（14）C]ADP发生转磷酸化反应，生成[（14）C]AMP和[（14）C]ATP。[（14）C]ATP仍紧密结合，只有通过使酶核苷酸复合物变性才能方便地回收。如果在存在绿脓菌素和[（3）H]AMP或[（32）P]P（i）的情况下照射菠菜膜，该酶可以作为与[（3）H]ADP或[（32）P]ADP的紧密复合物回收。证据表明，在光驱动的磷酸化反应中，AMP是比ADP更早的磷酸盐受体。这也表明AMP在光磷酸化中作为辅助因子起作用。
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