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烟草叶绿体中tRNAPro(UGG)、tRNATrp、tRNAfMet和tRNAGly(GCC)基因的位置和序列:tRNAGly的D茎中仅含有两个碱基对。

Locations and sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC): the tRNAGly contains only two base-pairs in the D stem.

作者信息

Ohme M, Kamogashira T, Shinozoki K, Sugiura M

出版信息

Nucleic Acids Res. 1984 Sep 11;12(17):6741-9. doi: 10.1093/nar/12.17.6741.

DOI:10.1093/nar/12.17.6741
PMID:6091037
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320113/
Abstract

The nucleotide sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC) have been determined. None of these genes contains an intron. One unusual feature is that the tRNAGly contains only two base-pairs (A-U, G-U) in the D stem. These four tRNA genes were located in the known physical map of tobacco chloroplast DNA. Hybridization analysis to chloroplast tRNA revealed that all four tRNA genes are transcribed in vivo.

摘要

已测定烟草叶绿体中tRNAPro(UGG)、tRNATrp、tRNAfMet和tRNAGly(GCC)基因的核苷酸序列。这些基因均不含有内含子。一个不同寻常的特征是,tRNAGly的D茎中仅含有两个碱基对(A-U、G-U)。这四个tRNA基因位于烟草叶绿体DNA的已知物理图谱中。对叶绿体tRNA的杂交分析表明,所有四个tRNA基因在体内均被转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/096f/320113/d5430792b200/nar00335-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/096f/320113/d5430792b200/nar00335-0109-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/096f/320113/d5430792b200/nar00335-0109-a.jpg

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本文引用的文献

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Proc Natl Acad Sci U S A. 1984 Jan;81(2):405-8. doi: 10.1073/pnas.81.2.405.
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Homology between chloroplast and prokaryotic initiator tRNA. Nucleotide sequence of spinach chloroplast methionine initiator tRNA.叶绿体与原核生物起始tRNA之间的同源性。菠菜叶绿体甲硫氨酸起始tRNA的核苷酸序列。
J Biol Chem. 1980 Oct 25;255(20):9981-4.
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9
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