Ohme M, Kamogashira T, Shinozoki K, Sugiura M
Nucleic Acids Res. 1984 Sep 11;12(17):6741-9. doi: 10.1093/nar/12.17.6741.
The nucleotide sequences of tobacco chloroplast genes for tRNAPro(UGG), tRNATrp, tRNAfMet and tRNAGly(GCC) have been determined. None of these genes contains an intron. One unusual feature is that the tRNAGly contains only two base-pairs (A-U, G-U) in the D stem. These four tRNA genes were located in the known physical map of tobacco chloroplast DNA. Hybridization analysis to chloroplast tRNA revealed that all four tRNA genes are transcribed in vivo.
已测定烟草叶绿体中tRNAPro(UGG)、tRNATrp、tRNAfMet和tRNAGly(GCC)基因的核苷酸序列。这些基因均不含有内含子。一个不同寻常的特征是,tRNAGly的D茎中仅含有两个碱基对(A-U、G-U)。这四个tRNA基因位于烟草叶绿体DNA的已知物理图谱中。对叶绿体tRNA的杂交分析表明,所有四个tRNA基因在体内均被转录。