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黑腹果蝇zeste基因座的分子克隆、种系转化及转录分析。

Molecular cloning, germ-line transformation, and transcriptional analysis of the zeste locus of Drosophila melanogaster.

作者信息

Gunaratne P H, Mansukhani A, Lipari S E, Liou H C, Martindale D W, Goldberg M L

出版信息

Proc Natl Acad Sci U S A. 1986 Feb;83(3):701-5. doi: 10.1073/pnas.83.3.701.

DOI:10.1073/pnas.83.3.701
PMID:3080747
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC322932/
Abstract

Approximately 170 kilobase pairs (kb) of contiguous DNA sequences derived from bands 3A3,4 of the Drosophila melanogaster X chromosome have been isolated by molecular cloning. Sequences required for the wild-type expression of the zeste locus are located within a 6-kb fragment of this chromosomal region, as shown by phenotypic rescue of zeste mutants in P element-mediated germ-line transformation. Expression of zeste is correlated with a 2.2-kb poly(A)+ RNA species transcribed at all postzygotic stages of Drosophila development. Many zeste alleles, including several producing neomorphic phenotypes, are not associated with detectable rearrangements of DNA.

摘要

通过分子克隆，已从果蝇X染色体的3A3、4带分离出约170千碱基对（kb）的连续DNA序列。如在P因子介导的种系转化中对zeste突变体的表型拯救所示，zeste基因座野生型表达所需的序列位于该染色体区域的一个6 kb片段内。zeste的表达与果蝇发育的所有合子后阶段转录的一种2.2 kb的聚腺苷酸加尾（poly(A)+）RNA种类相关。许多zeste等位基因，包括几个产生新形态表型的等位基因，与可检测到的DNA重排无关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/27d6cc6d9f30/pnas00307-0185-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/c9723f2a3593/pnas00307-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/bd69c6cbe7a7/pnas00307-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/4904472e2671/pnas00307-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/7383406aea27/pnas00307-0185-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/bb7efbb3c784/pnas00307-0185-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/1b3e0c6e8f39/pnas00307-0185-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/4072256e85bf/pnas00307-0185-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/27d6cc6d9f30/pnas00307-0185-g.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/c9723f2a3593/pnas00307-0184-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/bd69c6cbe7a7/pnas00307-0185-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/4904472e2671/pnas00307-0185-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/7383406aea27/pnas00307-0185-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/bb7efbb3c784/pnas00307-0185-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/1b3e0c6e8f39/pnas00307-0185-e.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/4072256e85bf/pnas00307-0185-f.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b91b/322932/27d6cc6d9f30/pnas00307-0185-g.jpg

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本文引用的文献

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Transcription of the white locus in Drosophila melanogaster.果蝇中白色基因座的转录。
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Unequal crossing-over associated with asymmetrical synapsis between nomadic elements in the Drosophila melanogaster genome.果蝇基因组中游牧元件之间不对称联会导致不等交换。
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Regena (Rga), a Drosophila homolog of the global negative transcriptional regulator CDC36 (NOT2) from yeast, modifies gene expression and suppresses position effect variegation.Regena（Rga）是酵母中全局负转录调节因子CDC36（NOT2）的果蝇同源物，它可修饰基因表达并抑制位置效应斑驳。
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