Peacock S, Brot N, Weissbach H
Biochem Biophys Res Commun. 1983 Jun 29;113(3):1018-25. doi: 10.1016/0006-291x(83)91100-2.
Using the plasmid pNF1337 as template, a mRNA preparation has been obtained that directs the in vitro synthesis of fMet-Val, the N-terminal dipeptide of the beta subunit of RNA polymerase. RNA polymerase holoenzyme specifically inhibits the mRNA-directed synthesis of fMet-Val showing that the autoregulation by RNA polymerase of beta, beta 1 synthesis is at the level of translation. L factor (nusA gene product) stimulates the synthesis of fMet-Val from a DNA template but not from mRNA. Rifampicin has no effect on the mRNA-directed synthesis of fMet-Val or the ability of RNA polymerase to inhibit fMet-Val synthesis.
以质粒pNF1337为模板，已获得一种mRNA制剂，它能指导体外合成甲硫酰 - 缬氨酸，即RNA聚合酶β亚基的N端二肽。RNA聚合酶全酶特异性抑制甲硫酰 - 缬氨酸的mRNA指导合成，表明RNA聚合酶对β、β1合成的自身调节是在翻译水平。L因子（nusA基因产物）刺激从DNA模板而非mRNA合成甲硫酰 - 缬氨酸。利福平对甲硫酰 - 缬氨酸的mRNA指导合成或RNA聚合酶抑制甲硫酰 - 缬氨酸合成的能力没有影响。
