Everett R D, Orr A, Elliott M
MRC Virology Unit, Glasgow, UK.
Nucleic Acids Res. 1991 Nov 25;19(22):6155-61. doi: 10.1093/nar/19.22.6155.
Herpes simplex virus type 1 (HSV-1) encodes five immediate early (IE) polypeptides. This paper reports the construction of a baculovirus vector which expresses large amounts of Vmw110, the product of IE gene 1. The expressed protein has been purified to near homogeneity and has a mobility on SDS polyacrylamide gels identical to that of Vmw110 produced during HSV-1 infection. Characterisation of its properties indicated that it forms dimers and perhaps higher order oligomers in solution and that the purified protein binds to both single stranded and double stranded calf thymus DNA cellulose columns. However, filter binding experiments were unable to detect any stable association of Vmw110 with DNA in solution.
单纯疱疹病毒1型（HSV - 1）编码五种立即早期（IE）多肽。本文报道了一种杆状病毒载体的构建，该载体可大量表达IE基因1的产物Vmw110。所表达的蛋白已被纯化至近乎同质，并且在SDS聚丙烯酰胺凝胶上的迁移率与HSV - 1感染期间产生的Vmw110相同。对其性质的表征表明，它在溶液中形成二聚体，可能还形成更高阶的寡聚体，并且纯化后的蛋白可与单链和双链小牛胸腺DNA纤维素柱结合。然而，滤膜结合实验未能检测到Vmw110在溶液中与DNA有任何稳定的结合。
