用于人类布鲁氏菌病诊断的外周血PCR检测中优化DNA扩增的策略。
Strategy for optimizing DNA amplification in a peripheral blood PCR assay used for diagnosis of human brucellosis.
作者信息
Morata P, Queipo-Ortuño M I, de Dios Colmenero J
机构信息
Department of Biochemistry and Molecular Biology, Málaga University, Málaga, Spain.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2443-6. doi: 10.1128/JCM.36.9.2443-2446.1998.
Abstract
We studied two of the possible factors which can interfere with specific DNA amplification in a peripheral-blood PCR assay used for the diagnosis of human brucellosis. We found that high concentrations of leukocyte DNA and heme compounds inhibit PCR. These inhibitors can be efficiently suppressed by increasing the number of washings to four or five and decreasing the amount of total DNA to 2 to 4 microg, thereby avoiding false-negative results.
摘要
我们研究了在用于诊断人类布鲁氏菌病的外周血聚合酶链反应（PCR）检测中可能干扰特异性DNA扩增的两个因素。我们发现高浓度的白细胞DNA和血红素化合物会抑制PCR。通过将洗涤次数增加到四到五次，并将总DNA量减少到2至4微克，可以有效抑制这些抑制剂，从而避免假阴性结果。
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本文引用的文献
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Rapid diagnosis of human brucellosis by peripheral-blood PCR assay.通过外周血PCR检测快速诊断人类布鲁氏菌病
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