Yiannikouris Alexandros, André Gwénaëlle, Poughon Laurent, François Jean, Dussap Claude-Gilles, Jeminet Georges, Bertin Gérard, Jouany Jean-Pierre
Alltech-France, 14 Place Marie-Jeanne Bassot, 92300 Levallois-Perret, France.
Biomacromolecules. 2006 Apr;7(4):1147-55. doi: 10.1021/bm050968t.
In a previous paper we reported that beta-D-glucans isolated from Saccharomyces cerevisiae could adsorb zearalenone, reduce its bioavailability in the digestive tract, and protect animals against its adverse effects. We have now investigated, in vitro, the kinetics of the interaction between other mycotoxins and beta-D-glucans from several sources at three pH values found along the digestive tract (3.0, 6.0, and 8.0). Acid and neutral conditions gave the highest affinity rates for aflatoxins B1 > deoxynivalenol > ochratoxin A and involved both the (1 --> 3)-beta-D-glucans and the (1 --> 6)-beta-D-glucans. Alkaline conditions, owing to their destructuring action on glucans, were favorable only for the adsorption of patulin. Using molecular mechanics, we found that hydroxyl, ketone, and lactone groups are involved in the formation of both hydrogen bonds and van der Waals interactions between aflatoxins B1, deoxynivalenol and patulin, and beta-D-glucans. Differences in the binding capacity of the mycotoxins are due to their specific physical and chemical characteristics.
在之前的一篇论文中，我们报道了从酿酒酵母中分离出的β-D-葡聚糖能够吸附玉米赤霉烯酮，降低其在消化道中的生物利用度，并保护动物免受其不良影响。我们现在已经在体外研究了其他霉菌毒素与来自几种来源的β-D-葡聚糖在消化道中发现的三个pH值（3.0、6.0和8.0）下的相互作用动力学。酸性和中性条件下，黄曲霉毒素B1>脱氧雪腐镰刀菌烯醇>赭曲霉毒素A的亲和率最高，（1→3）-β-D-葡聚糖和（1→6）-β-D-葡聚糖均参与其中。碱性条件由于其对葡聚糖的解构作用，仅有利于展青霉素的吸附。使用分子力学，我们发现羟基、酮基和内酯基参与了黄曲霉毒素B1、脱氧雪腐镰刀菌烯醇和展青霉素与β-D-葡聚糖之间氢键和范德华相互作用的形成。霉菌毒素结合能力的差异归因于它们特定的物理和化学特性。
