Vanlandingham Dana L, Tsetsarkin Konstantin, Klingler Kimberly A, Hong Chao, McElroy Kate L, Lehane Michael J, Higgs Stephen
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
Am J Trop Med Hyg. 2006 Apr;74(4):663-9.
The alphaviruses o'nyong nyong virus (ONNV) and chikungunya virus (CHIKV) provide a unique system to study the viral genes involved in vector specificity. ONNV infects both anopheline and culicine mosquitoes, whereas CHIKV infects only culicine mosquitoes. In this study, chimeric viruses were constructed that contained genes from both ONNV and CHIKV. These chimeras and previously described full-length infectious clones of ONNV and CHIKV were evaluated in Anopheles gambiae and Aedes aegypti mosquitoes. Virus derived from the infectious clones of ONNV and CHIKV retained the vector specificity of the parental viruses. All six of the chimeras were found to infect Ae. aegypti mosquitoes at high rates but only the chimera containing viral genes encoding all of the structural proteins of ONNV was able to infect An. gambiae mosquitoes. These data indicate that all of the viral structural proteins are necessary for ONNV to infect An. gambiae mosquitoes.
甲病毒属的奥尼永病毒（ONNV）和基孔肯雅病毒（CHIKV）为研究与媒介特异性相关的病毒基因提供了一个独特的系统。ONNV可感染按蚊和库蚊，而CHIKV仅感染库蚊。在本研究中，构建了包含ONNV和CHIKV基因的嵌合病毒。在冈比亚按蚊和埃及伊蚊中对这些嵌合体以及先前描述的ONNV和CHIKV全长感染性克隆进行了评估。源自ONNV和CHIKV感染性克隆的病毒保留了亲本病毒的媒介特异性。发现所有六个嵌合体都能以高感染率感染埃及伊蚊，但只有包含编码ONNV所有结构蛋白的病毒基因的嵌合体能够感染冈比亚按蚊。这些数据表明，ONNV感染冈比亚按蚊需要所有病毒结构蛋白。
