Unité d'Entomologie Médicale, Institut Pasteur de Dakar, Dakar, Sénégal.
Pole de virologie, Unité des Arbovirus et virus de Fièvres Hémorragiques, Institut Pasteur de Dakar, Dakar, Sénégal.
Emerg Microbes Infect. 2020 Feb 28;9(1):496-504. doi: 10.1080/22221751.2020.1730710. eCollection 2020.
The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of , , , and mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in , 28% in and 9.09% in . Viral particles in the saliva were confirmed infectious by C6/36 cell culture. was able to disseminate DKV only at 20 days post-infection. This study shows that mosquitoes are more competent than for DKV, while is not likely a competent vector.
mesoniviruses（MESOVs）属于新描述的 mesoniviridae 科（目：Nidovirales）。直到最近，在一项关于虫媒病毒出现的研究中，在塞内加尔东部的普通蚊子中检测到一种名为 DKV 的新型 MESOV，才首次报道了它们在塞内加尔的存在。实际上，这种新描述的 DKV 对其载体的媒介效能尚不清楚。因此，我们使用口服感染法评估了在塞内加尔采集的 、 、 和 蚊子对 DKV 的媒介效能。通过 RT-PCR 对整虫、腿/翅和唾液样本进行 DKV 检测，以评估感染、传播和传递率。通过感染 C6/36 细胞来确认唾液中病毒颗粒的感染力。在 、 和 中，病毒的感染率分别高达 95.45%、28%和 9.09%。通过 C6/36 细胞培养证实了唾液中的病毒颗粒具有传染性。仅在感染后 20 天， 就能够传播 DKV。本研究表明， 比 更适合 DKV，而 不太可能是一种有效的媒介。
