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使用两种快速检测方法对肠炎沙门氏菌进行初步鉴定。

Presumptive identification of Salmonella enterica using two rapid tests.

作者信息

Ruiz J, Varela M C, Sempere M A, Lopez M L, Gomez J, Oliva J

机构信息

Seccion de Microbiología, Hospital Virgen de la Arrixaca, Murcia, Spain.

出版信息

Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):649-51. doi: 10.1007/BF01975818.

Abstract

Two rapid test for early detection of Salmonella enterica, the cytochrome oxidase test and a fluorescence test, were evaluated in 1,200 colonies which had been isolated from human feces and cultured on MacConkey and Salmonella-Shigella media. Using the fluorescence test there were no false negative results (sensitivity 100%) and of 205 positive cases 62 did not correspond biochemically to salmonella (specificity 94.1%); 44 of these 62 were positive in the cytochrome oxidase test, raising the specificity to 98.2%. In conclusion, the combination of the two tests could be very useful due to the ease of performance, low cost and excellent results obtained.

摘要

对从人类粪便中分离并在麦康凯培养基和沙门氏菌-志贺氏菌培养基上培养的1200个菌落,评估了两种用于早期检测肠炎沙门氏菌的快速检测方法,即细胞色素氧化酶试验和荧光试验。使用荧光试验时,无假阴性结果(灵敏度100%),在205例阳性病例中,有62例在生化特性上与沙门氏菌不符(特异性94.1%);这62例中的44例在细胞色素氧化酶试验中呈阳性,使特异性提高到98.2%。总之,由于这两种检测方法操作简便、成本低且结果优异,联合使用可能非常有用。

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