Cabello C, Manjarrez M E, Olvera R, Villalba J, Valle L, Paramo I
Departamento de Investigación en Virología, Instituto Nacional de Enfermedades Respiratorias, Calzada de Tlalpan 4502, Mexico, DF.
Mem Inst Oswaldo Cruz. 2006 Feb;101(1):21-4. doi: 10.1590/s0074-02762006000100005. Epub 2006 Mar 30.
A locality in the district of Tlalpan, Mexico City, was selected in order to identify the viral agents in children younger than 5 years of age with acute respiratory infection (ARI). A total of 300 children were randomly selected and were included in this study for a period of 13 months. During this period nasopharyngeal exudates were collected for the isolation of viral agents. Monoclonal fluorescent antibodies were used for viral identification after cell culture. Viral infection was detected in 65% of the specimens. The respiratory syncytial virus (RSV) was the most common virus agent detected. Children required an average of two consultations during the study period. Two high incidence peaks were observed, one during the summer and the other during winter; the most frequent viruses during these seasons were influenza A and RSV, respectively. The largest number of viruses was isolated in the group of children between 1 and 2 years of age and in the group between 4 and 5 years of age. This study demonstrated the presence of ARI and of different viruses in a period of 13 months, as well as the most frequent viruses in children younger than 5 years of age from a community of Mexico City.
为了确定墨西哥城特拉尔潘区5岁以下患有急性呼吸道感染（ARI）儿童体内的病毒病原体，选取了该地区的一个地点。总共随机挑选了300名儿童，并将他们纳入本研究，为期13个月。在此期间，收集鼻咽分泌物以分离病毒病原体。细胞培养后，使用单克隆荧光抗体进行病毒鉴定。在65%的标本中检测到病毒感染。呼吸道合胞病毒（RSV）是检测到的最常见病毒病原体。在研究期间，儿童平均需要就诊两次。观察到两个高发高峰，一个在夏季，另一个在冬季；这些季节中最常见的病毒分别是甲型流感病毒和呼吸道合胞病毒。在1至2岁儿童组和4至5岁儿童组中分离出的病毒数量最多。这项研究证明了在13个月的时间里急性呼吸道感染和不同病毒的存在，以及墨西哥城一个社区中5岁以下儿童中最常见的病毒。
