中国湖北儿童急性呼吸道感染相关病毒和非典型细菌的检测
Detection of viruses and atypical bacteria associated with acute respiratory infection of children in Hubei, China.
作者信息
Wu Zegang, Li Yan, Gu Jian, Zheng Hongyun, Tong Yongqing, Wu Qing
机构信息
Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Wuhan, China.
出版信息
Respirology. 2014 Feb;19(2):218-224. doi: 10.1111/resp.12205. Epub 2013 Nov 20.
BACKGROUND AND OBJECTIVE
Acute respiratory infection is the major cause of disease and death in children, particularly in developing countries. However, the spectrum of pathogenic viruses and atypical bacteria that exist in many of these countries remains incompletely characterized. The aim of this study was to examine the spectrum of pathogenic viruses and atypical bacteria associated with acute respiratory infection in children under the age of 16.
METHODS
A total of 10 435 serum sera specimens were collected from hospitalized children presenting with acute respiratory infection symptoms. Indirect immunofluorescence assays were performed to detect immunoglobulin M antibodies against nine common pathogens: mycoplasma pneumonia, influenza virus B, respiratory syncytial virus, parainfluenza virus, adenovirus, influenza virus A, legionella pneumophila, coxiella burnetii and chamydophila pneumonia.
RESULTS
Of the 10 435 specimens examined, 7046 tested positive for at least one pathogen. Among all of the tested pathogens, mycoplasma pneumonia had the highest detection rate (56.9%). Influenza virus A and influenza virus B epidemics occurred during both winter and summer. The detection rate of respiratory syncytial virus and adenovirus was higher in spring. Cases of mixed infection were more complex: 4136 specimens (39.6%) tested positive for ≥2 pathogens. There were statistically significant difference in detection rates of mycoplasma pneumonia, influenza virus B, respiratory syncytial virus, parainfluenza virus, adenovirus, influenza virus A, legionella pneumophila and chamydophila pneumonia among different age groups (P < 0.05).
CONCLUSIONS
The most common pathogens causing acute respiratory infection among children in Hubei of China were mycoplasma pneumonia, influenza virus B and respiratory syncytial virus. The detection rates for each pathogen displayed specific seasonal and age group variations.
背景与目的
急性呼吸道感染是儿童发病和死亡的主要原因，在发展中国家尤为如此。然而，许多这些国家中存在的致病病毒和非典型细菌谱仍未完全明确。本研究的目的是检测16岁以下儿童急性呼吸道感染相关的致病病毒和非典型细菌谱。
方法
从出现急性呼吸道感染症状的住院儿童中总共收集了10435份血清标本。进行间接免疫荧光测定以检测针对九种常见病原体的免疫球蛋白M抗体：肺炎支原体、乙型流感病毒、呼吸道合胞病毒、副流感病毒、腺病毒、甲型流感病毒、嗜肺军团菌、伯氏考克斯氏体和肺炎衣原体。
结果
在检测的10435份标本中，7046份至少对一种病原体检测呈阳性。在所有检测的病原体中，肺炎支原体的检出率最高（56.9%）。甲型流感病毒和乙型流感病毒在冬季和夏季均有流行。呼吸道合胞病毒和腺病毒的检出率在春季较高。混合感染情况更为复杂：4136份标本（39.6%）对≥2种病原体检测呈阳性。不同年龄组之间肺炎支原体、乙型流感病毒、呼吸道合胞病毒、副流感病毒、腺病毒、甲型流感病毒、嗜肺军团菌和肺炎衣原体的检出率存在统计学显著差异（P < 0.05）。
结论
在中国湖北，引起儿童急性呼吸道感染最常见的病原体是肺炎支原体、乙型流感病毒和呼吸道合胞病毒。每种病原体的检出率呈现出特定的季节和年龄组差异。
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