Kondo S, Haishima Y, Hisatsune K
Department of Microbiology, School of Pharmaceutical Sciences, Josai University, Saitama, Japan.
FEMS Microbiol Lett. 1993 Jan 1;106(1):93-8. doi: 10.1111/j.1574-6968.1993.tb05940.x.
Fructose, a rarely occurring sugar constituent of Gram-negative bacterial lipopolysaccharides (LPS), is distributed ubiquitously in LPS of 01 Vibrio cholerae so far examined, but its location in LPS has not hitherto been elucidated. It was found that hydrazinolysis of LPS successfully affords a derivative retaining virtually all the fructose of intact LPS, but no ester-bound phosphate. Structural analysis carried out on the LPS derivative prepared by the hydrazinolysis of R-type LPS isolated from a rough mutant strain (NIH 41R) of 01 V. cholerae NIH 41 (Ogawa) revealed that the fructose is present as a non-reducing terminal residue bound to position C-6 of a glucose residue in the core region. This finding is considered to exclude the possibility that, in the LPS of 01 V. cholerae, the fructose is present in the region of the inner core in place of 2-keto-3-deoxyoctonate.
果糖是革兰氏阴性菌脂多糖（LPS）中一种罕见的糖成分，在迄今检测的01群霍乱弧菌的LPS中普遍存在，但它在LPS中的位置尚未阐明。研究发现，LPS经肼解可成功得到一种衍生物，该衍生物几乎保留了完整LPS中的所有果糖，但没有酯键结合的磷酸基团。对从01群霍乱弧菌NIH 41（小川型）的粗糙突变株（NIH 41R）分离出的R型LPS进行肼解制备的LPS衍生物进行结构分析，结果显示果糖以非还原末端残基的形式存在，与核心区域葡萄糖残基的C-6位相连。这一发现被认为排除了在01群霍乱弧菌的LPS中，果糖存在于内核区域替代2-酮-3-脱氧辛酸的可能性。
