Kondo S, Haishima Y, Hisatsune K
Department of Microbiology, School of Pharmaceutical Sciences Josai University, Saitama, Japan.
FEMS Microbiol Lett. 1990 Mar 1;56(1-2):155-8. doi: 10.1016/0378-1097(90)90142-d.
Phosphorylated 2-keto-3-deoxyoctonate (KDO) has been detected in the strong-acid hydrolysates of lipopolysaccharides (LPS) of family Vibrionaceae including Vibrio cholerae. Structural analysis of LPS isolated from a rough mutant of non-01 V. cholerae 05 by dephosphorylation, periodate oxidation and methylation analysis revealed that the inner core region of the LPS molecule contains only one mole of KDO in contrast to enteric Gram-negative bacterial LPS, and that the phosphate group on the KDO molecule resides in the C4 position, while the site of binding of KDO to heptose, a constituent of the distal part of the inner core region, is the C5 position as in the enteric bacterial LPS.
