Rodrigues Lígia R, Teixeira José A, van der Mei Henny C, Oliveira Rosário
Centro de Engenharia Biológica, Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
Colloids Surf B Biointerfaces. 2006 Apr 15;49(1):79-86. doi: 10.1016/j.colsurfb.2006.03.003. Epub 2006 Apr 17.
Isolation and identification of key components of the crude biosurfactant produced by Lactococcus lactis 53 was studied. Fractionation was achieved by hydrophobic interaction chromatography which allowed the isolation of a fraction rich in glycoproteins. Molecular (by Fourier transform infrared spectroscopy) and elemental compositions (by X-ray photoelectron spectroscopy) were determined. Critical micelle concentration achieved for the isolated fraction was 14 g/l, allowing for a surface tension value of 36 mJ/m(2). Moreover, the isolated fraction, stable to pH changes between 5 and 9, was found to be an anti-adhesive and antimicrobial agent against several bacterial and yeast strains isolated from explanted voice prostheses, even at low concentrations. Further purification steps should be carefully analyzed as each purification step will increase the costs and decreases the amounts of biosurfactants recovered.
对乳酸乳球菌53产生的粗生物表面活性剂关键成分的分离与鉴定进行了研究。通过疏水相互作用色谱法进行分级分离，从而分离出富含糖蛋白的级分。测定了其分子组成（通过傅里叶变换红外光谱）和元素组成（通过X射线光电子能谱）。分离出的级分的临界胶束浓度为14 g/l，表面张力值为36 mJ/m²。此外，发现该分离级分在pH值5至9之间变化时稳定，即使在低浓度下，对从植入的语音假体分离出的几种细菌和酵母菌株也是一种抗粘附和抗菌剂。由于每个纯化步骤都会增加成本并减少回收的生物表面活性剂的量，因此应仔细分析进一步的纯化步骤。
