China Pharmaceutical University, Nanjing, PR China.
Int J Biol Macromol. 2010 May 1;46(4):451-7. doi: 10.1016/j.ijbiomac.2010.02.006. Epub 2010 Feb 12.
Two low-molecular-weight polysaccharides, GLP(L)1 and GLP(L)2, purified from a crude Ganoderma lucidum polysaccharide preparation GLPP were investigated for their physicochemical properties, structure characterization and antioxidant activities. The results indicated that GLP(L)1 was a glucan with an average molecular weight of 5.2kDa, while GLP(L)2 was composed of glucose, galactose and mannose in a ratio of 29:1.8:1.0 with the average molecular weight of 15.4kDa. GLP(L)1 and GLP(L)2 had similar structure characteristic which contained linkages such as -->3)-Glcp-(1-->, -->4)-Glcp-(1-->, -->6)-Glcp-(1-->, -->3,6)-Glcp-(1-->, and -->4,6)-Glcp-(1--> in the percentage ratio of 21.9:20.3:23.7:24.0:3.7 and 23.0:34.6:7.0:14.1:3.0 in the backbone or branches, respectively. Antioxidant results showed that both GLP(L)1 and GLP(L)2 exhibited antioxidant activities while GLP(L)1 was more effective in free radicals scavenging and Fe(2+) chelating. Low-molecular-weight polysaccharide seems to play an important role in the exploration of natural antioxidants in food industry and pharmaceuticals.
两种从灵芝粗多糖 GLPP 中分离得到的低分子量多糖 GLP(L)1 和 GLP(L)2，对其理化性质、结构特征和抗氧化活性进行了研究。结果表明，GLP(L)1 是一种平均分子量为 5.2kDa 的葡聚糖，而 GLP(L)2 由葡萄糖、半乳糖和甘露糖组成，摩尔比为 29:1.8:1.0，平均分子量为 15.4kDa。GLP(L)1 和 GLP(L)2 具有相似的结构特征，含有 -->3)-Glcp-(1-->, -->4)-Glcp-(1-->, -->6)-Glcp-(1-->, -->3,6)-Glcp-(1-->, 和 -->4,6)-Glcp-(1-->等糖苷键，其百分比分别为 21.9:20.3:23.7:24.0:3.7 和 23.0:34.6:7.0:14.1:3.0。抗氧化结果表明，GLP(L)1 和 GLP(L)2 均具有抗氧化活性，而 GLP(L)1 在自由基清除和 Fe(2+)螯合方面的效果更为显著。低分子量多糖似乎在食品工业和制药领域天然抗氧化剂的开发中发挥着重要作用。
