Yuan H, van Blitterswijk C A, de Groot K, de Bruijn J D
BMTI, Twente University, Enschede, The Netherlands.
J Biomed Mater Res A. 2006 Jul;78(1):139-47. doi: 10.1002/jbm.a.30707.
Physicochemical modification could implement synthetic materials into osteoinductive materials, which induce bone formation in nonosseous tissues. We hereby studied the relevance between the osteogenic capacities of osteoinductive materials in nonosseous tissues and in osseous sites. Biphasic calcium phosphate ceramic (BCP) and hydroxyapatite ceramic (HA) were implanted in femoral muscles and femoral cortical bone of dogs for 7, 14, 21, 30, 45, 60, 90, 180, and 360 days, respectively. Two dogs were used in each time point. In each dog, four cylinders (phi5x6 mm) per material were implanted in femoral muscles and 2 cylinders (phi5x6 mm) per material in femoral cortical bone. The harvested samples were processed for both histological and histomorphometric analyses. Bone was observed in BCP implanted in femoral muscles since day 30, while in HA since day 45. Quantitatively, more bone was formed in BCP than in HA at each time point after day 30 (p<0.05). The earlier and more bone formed in BCP than in HA suggests BCP a higher osteoinductive potential than HA in muscle. In femoral cortical bone defects, a bridge of bone in the defect with BCP was observed at day 21, while with HA at day 30. At days 14, 21, and 30, significantly more bone was formed in BCP than in HA (p<0.05). The results herein show that osteogenic capacities of osteoinductive materials in nonosseous tissues and osseous sites are correlated: the higher the osteoinductive potential of the material, the faster the bone repair.
物理化学修饰可将合成材料转变为骨诱导材料，这类材料能在非骨组织中诱导骨形成。我们在此研究了骨诱导材料在非骨组织和骨部位的成骨能力之间的相关性。将双相磷酸钙陶瓷（BCP）和羟基磷灰石陶瓷（HA）分别植入犬的股四头肌和股皮质骨中7、14、21、30、45、60、90、180和360天。每个时间点使用两只犬。在每只犬中，每种材料的四个圆柱体（直径5×6毫米）植入股四头肌，每种材料的两个圆柱体（直径5×6毫米）植入股皮质骨。对收获的样本进行组织学和组织形态计量学分析。自第30天起在植入股四头肌的BCP中观察到骨形成，而HA则自第45天起观察到骨形成。定量分析显示，在第30天之后的每个时间点，BCP中形成的骨比HA中更多（p<0.05）。BCP比HA更早且更多地形成骨，表明BCP在肌肉中的骨诱导潜力高于HA。在股皮质骨缺损中，在第21天观察到BCP植入处缺损部位有骨桥形成，而HA则在第30天观察到。在第14、21和30天，BCP中形成的骨明显多于HA（p<0.05）。本文结果表明，骨诱导材料在非骨组织和骨部位的成骨能力是相关的：材料的骨诱导潜力越高，骨修复速度越快。
