Gonzales-Alvarado Antony Cristhian, Cardoso Jean Carlos
Master Science Graduate Program of Plant Production and Associated Bioprocesses, Center of Agricultural Sciences, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
Laboratory of Plant Physiology and Tissue Culture, Department of Biotechnology, Plant and Animal Production, Center of Agricultural Sciences, Federal University of São Carlos, Araras 13600-970, SP, Brazil.
Plants (Basel). 2024 Oct 11;13(20):2842. doi: 10.3390/plants13202842.
In vitro cultivation of could contribute to the cloning of superior genotypes. Studies of factors affecting micropropagation are needed, such as the interaction with light-emitting diodes (LEDs) and plant growth regulators added to the culture media. This study aimed at better understanding the effects of spectra on the development and physiology of melaleuca cultivated in vitro, as well as the interaction of LEDs with the main cytokinin used in micropropagation, N6-Benzylaminopurine (6-BAP). 6-BAP, spectra, and their interaction had a significant effect on most of the variables analyzed, altering the in vitro development and chlorophyll concentrations in the plants, as well as changing different variables in the culture medium, such as pH, EC, and levels of Ca, Mg, and P, and nutrient accumulation in the shoots. The results demonstrate that the main effects of adding BAP to the in vitro cultivation of melaleuca are an increase in the number of shoots, which resulted in greater fresh and dry masses; a reduction in height and chlorophyll content; complete inhibition of adventitious rooting; higher consumption of Mg, and lower consumption of Ca and P from the culture medium; higher content of Fe, and lower content of P, S, Mn, Cu and B in the in vitro shoot tissues.
[植物名称]的离体培养有助于优良基因型的克隆。需要研究影响微繁殖的因素，例如与发光二极管（LED）的相互作用以及添加到培养基中的植物生长调节剂。本研究旨在更好地了解光谱对离体培养的千层树发育和生理的影响，以及LED与微繁殖中使用的主要细胞分裂素N6-苄基腺嘌呤（6-BAP）的相互作用。6-BAP、光谱及其相互作用对所分析的大多数变量都有显著影响，改变了植物的离体发育和叶绿素浓度，也改变了培养基中的不同变量，如pH值、电导率以及钙、镁和磷的含量，还有茎中的养分积累。结果表明，在千层树离体培养中添加BAP的主要作用是增加茎的数量，从而导致更大的鲜重和干重；降低高度和叶绿素含量；完全抑制不定根形成；增加对镁的消耗，减少对培养基中钙和磷的消耗；离体茎组织中铁含量较高，磷、硫、锰、铜和硼含量较低。
