多基因实时PCR检测肺癌患者外周血中的循环肿瘤细胞
Multigene real-time PCR detection of circulating tumor cells in peripheral blood of lung cancer patients.
作者信息
Hayes Dawn C, Secrist Heather, Bangur Chaitanya S, Wang Tongtong, Zhang Xinqun, Harlan Dianne, Goodman Gary E, Houghton Raymond L, Persing David H, Zehentner Barbara K
机构信息
GSK-Biologicals-Seattle, 1900 9th Avenue, Suite 1100, Seattle, WA 98101 USA.
出版信息
Anticancer Res. 2006 Mar-Apr;26(2B):1567-75.
BACKGROUND
CLCA2, HMGB3, L587S and ASH1 were identified in lung cancer tissues using genetic subtraction, microarray and quantitative PCR, and found to be specific and complementary for detection of non-small cell lung carcinoma (NSCLC) and small cell lung carcinoma (SCLC).
MATERIALS AND METHODS
A real-time RT-PCR assay, simultaneously detecting four genes, was developed and tested on lung cancer specimens.
RESULTS
Twenty-two out of 24 adenocarcinomas, 18/18 squamous, 4/5 large cell, 2/2 small cell and 2/2 bronchoalveolar/neuroendocrine cancer tissue samples tested positive. Specificity was demonstrated by evaluation of 194 other tumor and corresponding normal tissues. Circulating tumor cells in the peripheral blood of 49/108 lung cancer patient samples tested positive, and general correlations of multigene expression signals to disease status were observed. Changes in multigene expression during treatment and disease recurrence in individual patients could be detected.
CONCLUSION
These data indicate the diagnostic and prognostic utility of a multigene real-time RT-PCR assay to detect tumor cells in the peripheral blood of lung cancer patients.
背景
通过基因消减、微阵列和定量PCR技术在肺癌组织中鉴定出CLCA2、HMGB3、L587S和ASH1基因，发现它们对非小细胞肺癌（NSCLC）和小细胞肺癌（SCLC）的检测具有特异性和互补性。
材料与方法
开发了一种同时检测四个基因的实时RT-PCR检测方法，并在肺癌标本上进行测试。
结果
24例腺癌组织样本中有22例、18例鳞癌组织样本中的18例、5例大细胞癌组织样本中的4例、2例小细胞癌组织样本中的2例以及2例支气管肺泡/神经内分泌癌组织样本检测呈阳性。通过对194例其他肿瘤及相应正常组织的评估证实了该检测方法的特异性。108例肺癌患者样本中有49例患者外周血中的循环肿瘤细胞检测呈阳性，并且观察到多基因表达信号与疾病状态之间存在总体相关性。可以检测到个体患者在治疗和疾病复发过程中多基因表达的变化。
结论
这些数据表明多基因实时RT-PCR检测方法在检测肺癌患者外周血中肿瘤细胞方面具有诊断和预后价值。
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