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使用手持风扇快速干燥细针穿刺涂片:对周转时间和染色质量的影响

Fast drying of Fine Needle Aspiration slides using a hand held fan: impact on turn around time and staining quality.

作者信息

Baig Mirza A, Fathallah Lamia, Feng Jining, Husain Mujtaba, Grignon David G, Al-Abbadi Mousa A

机构信息

Department of Pathology, Wayne State University/Detroit Medical Center, Detroit, MI, USA.

出版信息

Cytojournal. 2006 Apr 19;3:12. doi: 10.1186/1742-6413-3-12.

DOI:10.1186/1742-6413-3-12
PMID:16623950
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1481514/
Abstract

To analyze the impact of using a hand held fan to speed the air-drying process during immediate adequacy evaluation of Fine Needle Aspirations. The effect on turn around time and staining quality is evaluated. Two mirror image air-dried smears for each pass were prepared. One was subjected to a small hand-held fan with a fan diameter of 7 cm held an average distance of 3 to 5 cm from the slide. The other smear was left to dry without a fan. A total of 93 consecutive pairs were evaluated over a 2-month duration. The average time needed for air-drying using the fan was 73 seconds (range 10-300 seconds, standard error 6.986), while it was 200 seconds (range 15-645 seconds, standard error 17.799) for those without fan. This difference was statistically significant (p < 0.001). Smears were then evaluated for single cells, cell clusters and background material and no appreciable difference in stain quality was noted between the 2 groups. The use of a small hand-held fan for air-drying shortened the drying time for FNA adequacy by an average of 127 seconds (63% time reduction) for each pass. The quality of staining was comparable. Using a fan is highly recommended.

摘要

分析在细针穿刺抽吸物即时充分性评估期间使用手持风扇加速空气干燥过程的影响。评估其对周转时间和染色质量的影响。每次操作制备两张镜像空气干燥涂片。一张涂片使用直径7厘米的小型手持风扇,风扇距载玻片平均距离为3至5厘米进行吹干。另一张涂片不使用风扇自然干燥。在两个月的时间内共评估了93组连续配对样本。使用风扇进行空气干燥的平均时间为73秒(范围10 - 300秒,标准误差6.986),而不使用风扇的平均时间为200秒(范围15 - 645秒,标准误差17.799)。这种差异具有统计学意义(p < 0.001)。然后对涂片的单个细胞、细胞簇和背景物质进行评估,两组之间在染色质量上未发现明显差异。使用小型手持风扇进行空气干燥,每次操作可将FNA充分性评估的干燥时间平均缩短127秒(时间减少63%)。染色质量相当。强烈建议使用风扇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ebb8d0fa3af5/1742-6413-3-12-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/226661a910d5/1742-6413-3-12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ed8ac6636ba7/1742-6413-3-12-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/4321e8b83fea/1742-6413-3-12-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ab70f40bc57d/1742-6413-3-12-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ebb8d0fa3af5/1742-6413-3-12-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/226661a910d5/1742-6413-3-12-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ed8ac6636ba7/1742-6413-3-12-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/4321e8b83fea/1742-6413-3-12-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ab70f40bc57d/1742-6413-3-12-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7a0/1481514/ebb8d0fa3af5/1742-6413-3-12-5.jpg

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