Cabria María Teresa, Rubines Jonathan, Gómez-Moliner Benjamín, Zardoya Rafael
Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, CSIC, José Gutiérrez Abascal, 2, 28006 Madrid, Spain.
Gene. 2006 Jun 21;375:1-13. doi: 10.1016/j.gene.2006.01.038. Epub 2006 Mar 17.
The nucleotide sequences of the complete mitochondrial genome and nine partial nuclear genes of the Pyrenean desman (Galemys pyrenaicus) were determined in order to establish the relative phylogenetic position of this species at different taxonomic levels within the placental tree. Phylogenetic relationships of desman within the family Talpidae were inferred based on complete mitochondrial cytochrome b gene nucleotide sequence data. The Pyrenean desman was unambiguously recovered as sister group of the Russian desman (Desmana moschata) confirming the monophyly of the subfamily Desmaninae. However, phylogenetic relationships among major lineages within the Talpidae could not be confidently resolved. Phylogenetic analyses based on mitochondrial (at the amino acid level) and nuclear (at the nucleotide level) sequence data sets confidently placed desman within the Eulipotyphla (that also included moles, shrews, and hedgehogs), and partially recovered placental interordinal relationships. The monophyly of Laurasiatheria (including Eulipotyphla, Chiroptera, Carnivora, Pholidota, Perissodactyla, and Cetartiodactyla) was strongly supported. Mitochondrial amino acid sequences of Pholidota (pangolins) were found to bias phylogenetic inferences due to long-branch attraction effects. A Bayesian inference based on a combined mitochondrial and nuclear data set without Pholidota arrived at an almost fully resolved tree that supported the basal position of Eulipotyphla within Laurasiatheria.
为了确定比利牛斯水鼹（Galemys pyrenaicus）在胎盘类谱系中不同分类水平上的相对系统发育位置，我们测定了其完整线粒体基因组和九个部分核基因的核苷酸序列。基于完整的线粒体细胞色素b基因核苷酸序列数据，推断了水鼹科内水鼹的系统发育关系。比利牛斯水鼹被明确地确定为俄罗斯水鼹（Desmana moschata）的姐妹群，证实了水鼹亚科的单系性。然而，水鼹科内主要谱系之间的系统发育关系无法得到可靠的解析。基于线粒体（氨基酸水平）和核（核苷酸水平）序列数据集的系统发育分析明确地将水鼹置于真盲缺目（其中还包括鼹鼠、鼩鼱和刺猬）内，并部分恢复了胎盘类目间的关系。劳亚兽总目（包括真盲缺目、翼手目、食肉目、鳞甲目、奇蹄目和鲸偶蹄目）的单系性得到了有力支持。由于长枝吸引效应，发现鳞甲目（穿山甲）的线粒体氨基酸序列会使系统发育推断产生偏差。基于一个不包括鳞甲目的线粒体和核数据集的贝叶斯推断得出了一棵几乎完全解析的树，支持了真盲缺目在劳亚兽总目内的基部位置。
