使用长寿命发光探针进行漫射光学成像的可行性。
Feasibility of diffuse optical imaging with long-lived luminescent probes.
作者信息
Apreleva Sofia V, Wilson David F, Vinogradov Sergei A
机构信息
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
出版信息
Opt Lett. 2006 Apr 15;31(8):1082-4. doi: 10.1364/ol.31.001082.
Abstract
Long-lived near-infrared phosphors with high quantum yields have recently become available, making it possible to image oxygen distributions in tissue in three dimensions. By numerical simulations we demonstrate that, by using phosphorescent probes with appropriate oxygen quenching constants, one can image hypoxic phantoms in scattering media with adequate spatial resolution, employing simple time-gated measurements. The approach developed will guide experimental imaging of phosphorescence lifetime and oxygen pressure in living tissue.
摘要
具有高量子产率的长寿命近红外磷光体近来已可获得，这使得对组织中的氧分布进行三维成像成为可能。通过数值模拟，我们证明，通过使用具有适当氧猝灭常数的磷光探针，利用简单的时间门控测量，就能够以足够的空间分辨率对散射介质中的缺氧模型进行成像。所开发的方法将指导对活组织中磷光寿命和氧压的实验成像。
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