Veena Krishnamurthy, Shanthi Palanivelu, Sachdanandam Panchanatham
Department of Medical Biochemistry, Dr. A.L. Mudaliar Post-Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai 600 113, India.
Chem Biol Interact. 2006 May 15;161(1):69-78. doi: 10.1016/j.cbi.2006.03.003. Epub 2006 Mar 13.
Breast cancer is one of the most common cancers in women of developed and developing countries. Lipids, lipoproteins and lipid-metabolizing enzymes have been associated with the risk of breast cancer. Kalpaamruthaa (KA) is a modified Siddha preparation, which contains Semecarpus anacardium Linn. (SA), Emblica officinalis (EO) and honey.
The present study was embarked to study the variations in lipids, lipid-metabolizing enzymes and lipoproteins in cancerous animals and the effect of KA on the lipid metabolism.
Breast cancer was induced in rats by administrating 7,12-dimethylbenz(a)anthracene orally (25 mg/kg body weight). After 90 days of induction, KA (300 mg/kg body weight) and SA (200 mg/kg body weight) were administered for 14 days, by gastric intubations. The levels of lipids and lipid-metabolizing enzymes were analysed in control and experimental animals.
The increased levels of total cholesterol, free cholesterol, phospholipids, triglycerides and free fatty acids and decreased levels of ester cholesterol in plasma, liver and kidney found in cancer suffering animals were reverted back to near normal levels on treatment with KA and SA. In mammary carcinoma bearing animals, the activities of total lipase, cholesterol ester synthase, and cholesterol ester hydrolase were significantly (p < 0.05) increased whereas lipoprotein lipase and lecithin cholesterol-acyl transferase were decreased. The levels of very low-density lipoprotein (VLDL) and low-density lipoprotein (LDL) were increased and the level of high-density lipoprotein (HDL) was decreased. These alterations were recouped back upon treatment with KA as well as SA when compared to cancer animals. The effects of KA were found to be more effective than SA. No significant alterations were observed in herbal preparation control animals when compared to control animals.
乳腺癌是发达国家和发展中国家女性中最常见的癌症之一。脂质、脂蛋白和脂质代谢酶与乳腺癌风险相关。Kalpaamruthaa（KA）是一种改良的悉达（Siddha）制剂，含有印度楝（Semecarpus anacardium Linn.，SA）、余甘子（Emblica officinalis，EO）和蜂蜜。
本研究旨在研究患癌动物脂质、脂质代谢酶和脂蛋白的变化以及KA对脂质代谢的影响。
通过口服7,12-二甲基苯并(a)蒽（25mg/kg体重）诱导大鼠患乳腺癌。诱导90天后，通过胃插管给予KA（300mg/kg体重）和SA（200mg/kg体重），持续14天。分析对照动物和实验动物的脂质和脂质代谢酶水平。
患癌动物血浆、肝脏和肾脏中总胆固醇、游离胆固醇、磷脂、甘油三酯和游离脂肪酸水平升高，酯胆固醇水平降低，用KA和SA治疗后恢复到接近正常水平。在患乳腺癌的动物中，总脂肪酶、胆固醇酯合成酶和胆固醇酯水解酶的活性显著（p<0.05）升高，而脂蛋白脂肪酶和卵磷脂胆固醇酰基转移酶活性降低。极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）水平升高，高密度脂蛋白（HDL）水平降低。与患癌动物相比，用KA和SA治疗后这些改变得到恢复。发现KA的效果比SA更有效。与对照动物相比，草药制剂对照动物未观察到明显改变。
