Gea-Botella Sara, Moreno-Chamba Bryan, de la Casa Laura, Salazar-Bermeo Julio, Martí Nuria, Martínez-Madrid María Concepción, Valero Manuel, Saura Domingo
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE), Universidad Miguel Hernández de Elche, 03202 Alicante, Spain.
Departamento de Agroquímica y Medio Ambiente, Universidad Miguel Hernández de Elche, 03312 Alicante, Spain.
Pharmaceutics. 2021 Nov 8;13(11):1898. doi: 10.3390/pharmaceutics13111898.
Persimmon ( Thunb.) fruits are a remarkable source of carotenoids, which have shown protective effects against UV radiation in bacteria, fungi, algae, and plants. The aim of this study was to analyze the photoprotection provided by an acetone extract, rich in carotenoids and obtained from byproducts derived from the persimmon juice industry, against UV-induced cell death in the keratinocyte HaCaT cell line. For this purpose, the cytotoxicity and phototoxicity of carotenoid extract, as well as its intracellular reactive oxygen species (ROS) scavenging and anti-adhesive activities towards HaCaT cells, were evaluated. The in vitro permeation test provided information about the permeability of the carotenoid extract. Persimmon extracts, rich in carotenoids (PEC), were absorbed by HaCaT keratinocyte cells, which reduced the UV-induced intracellular ROS production in treated cells. Thus, PEC exerted a photoprotective and regenerative effect on UV-irradiated HaCaT cells, and this protection was UV dose-dependent. No cytotoxic effect was observed in HaCaT cultures at the concentration tested. PEC treatment also stimulated the adhesion capacity of skin microbiome to HaCaT cells, while exhibiting a significant anti-adhesive activity against all tested pathogens. In conclusion, PEC showed potential for use as a functional ingredient in skin-care products.
柿（柿科）果实是类胡萝卜素的重要来源，类胡萝卜素已在细菌、真菌、藻类和植物中显示出对紫外线辐射的保护作用。本研究的目的是分析一种富含类胡萝卜素的丙酮提取物的光保护作用，该提取物从柿汁工业的副产品中获得，用于对抗角质形成细胞HaCaT细胞系中紫外线诱导的细胞死亡。为此，评估了类胡萝卜素提取物的细胞毒性和光毒性，以及其对HaCaT细胞的细胞内活性氧（ROS）清除和抗粘附活性。体外渗透试验提供了有关类胡萝卜素提取物渗透性的信息。富含类胡萝卜素的柿提取物（PEC）被HaCaT角质形成细胞吸收，这减少了紫外线诱导的处理细胞内ROS的产生。因此，PEC对紫外线照射的HaCaT细胞发挥了光保护和再生作用，且这种保护作用是紫外线剂量依赖性的。在所测试的浓度下，未在HaCaT培养物中观察到细胞毒性作用。PEC处理还刺激了皮肤微生物群对HaCaT细胞的粘附能力，同时对所有测试病原体表现出显著的抗粘附活性。总之，PEC显示出作为护肤品功能成分的应用潜力。
