Morgan C A, Herman N, White P A, Vesey G
BTF Pty Ltd, PO Box 599, North Ryde BC, NSW 1670, Australia.
J Microbiol Methods. 2006 Aug;66(2):183-93. doi: 10.1016/j.mimet.2006.02.017. Epub 2006 May 2.
The preservation of micro-organisms by different drying methodologies has been used for decades. Freeze drying in particular is the preferred method for transporting and storing vast culture collections of micro-organism strain types. The literature on drying and preserving micro-organisms is extensive, but is often specific to one particular strain. This review attempts to draw some similar concepts and findings together in one paper, to compare different drying techniques, with specific reference to microorganisms. The main topics covered are cell growth phases and concentration, inducing drying tolerance in microbial cells, drying methods, rehydration of dried cells and packaging and storage conditions. Also, particular attention has been paid to the use of freeze drying and the protective matrices used to improve microbial cell viability after drying.
几十年来，人们一直使用不同的干燥方法来保存微生物。特别是冷冻干燥，它是运输和储存大量微生物菌株类型培养物的首选方法。关于微生物干燥和保存的文献浩如烟海，但往往只针对某一特定菌株。本综述试图将一些相似的概念和研究结果汇总在一篇论文中，比较不同的干燥技术，特别提及微生物。涵盖的主要主题包括细胞生长阶段和浓度、诱导微生物细胞的干燥耐受性、干燥方法、干燥细胞的复水以及包装和储存条件。此外，还特别关注了冷冻干燥的应用以及用于提高干燥后微生物细胞活力的保护基质。
