通过气相色谱-电子轰击质谱法对植物中五环三萜类化合物进行去重复化分析。
Dereplication of pentacyclic triterpenoids in plants by GC-EI/MS.
作者信息
Gu Jian-Qiao, Wang Yuehong, Franzblau Scott G, Montenegro Gloria, Timmermann Barbara N
机构信息
Department of Pharmacology and Toxicology, Division of Medicinal and Natural Products Chemistry, College of Pharmacy, University of Arizona, Tucson, AZ 85721, USA.
出版信息
Phytochem Anal. 2006 Mar-Apr;17(2):102-6. doi: 10.1002/pca.892.
Three common plant-derived pentacyclic triterpenoids, oleanolic acid (1), betulinic acid (2) and ursolic acid (3), have been found to exhibit moderate anti-tubercular activity in a microplate alamar blue assay. In order to facilitate the discovery of novel anti-tubercular leads with diverse chemical structures, a new and rapid GC-EI/MS method was developed simultaneously and unambiguously to dereplicate 1-3 as their methyl esters with limits of detection of 25.6, 26.9 and 26.8 ng, respectively.
已发现三种常见的植物源五环三萜类化合物，齐墩果酸（1）、桦木酸（2）和熊果酸（3），在微孔板alamar蓝试验中表现出中等抗结核活性。为了便于发现具有不同化学结构的新型抗结核先导化合物，同时开发了一种新的快速气相色谱-电子轰击质谱（GC-EI/MS）方法，该方法能明确地将1-3去重复识别为它们的甲酯，检测限分别为25.6、26.9和26.8纳克。
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